Amphiregulin Is a Novel Growth Factor Involved in Normal Bone Development and in the Cellular Response to Parathyroid Hormone Stimulation

Qin, Ling, Tamasi, Joseph, Raggatt, Liza, Li, Xin, Feyen, Jean H. M., DiCicco-Bloom|, Emanuel and Partridge, Nicola C. (2005) Amphiregulin Is a Novel Growth Factor Involved in Normal Bone Development and in the Cellular Response to Parathyroid Hormone Stimulation. Journal of Biological Chemistry, 280 5: 3974-3981. doi:10.1074/jbc.M409807200

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ160932_OA.pdf Full text (open access) application/pdf 382.42KB 0

Author Qin, Ling
Tamasi, Joseph
Raggatt, Liza
Li, Xin
Feyen, Jean H. M.
DiCicco-Bloom|, Emanuel
Partridge, Nicola C.
Title Amphiregulin Is a Novel Growth Factor Involved in Normal Bone Development and in the Cellular Response to Parathyroid Hormone Stimulation
Journal name Journal of Biological Chemistry   Check publisher's open access policy
ISSN 0021-9258
1083-351X
Publication date 2005-02-04
Year available 2005
Sub-type Article (original research)
DOI 10.1074/jbc.M409807200
Open Access Status File (Publisher version)
Volume 280
Issue 5
Start page 3974
End page 3981
Total pages 8
Place of publication Bethesda MD, USA
Publisher American Society for Biochemistry & Molecular Biology
Collection year 2005
Language eng
Subject 110314 Orthopaedics
110306 Endocrinology
Abstract Parathyroid hormone (PTH) is the major mediator of calcium homeostasis and bone remodeling and is now known to be an effective drug for osteoporosis treatment. Yet the mechanisms responsible for its functions in bone are largely unknown. Here we report that the expression of amphiregulin (AR), a member of the epidermal growth factor (EGF) family, is rapidly and highly up-regulated by PTH in several osteoblastic cell lines and bone tissues. Other osteotropic hormones (1{alpha},25-dihydroxyvitamin D3 and prostaglandin E2) also strongly stimulate AR expression. We found all EGF-like ligands and their receptors are expressed in osteoblasts, but AR is the only member that is highly regulated by PTH. Functional studies demonstrated that although AR is a potent growth factor for preosteoblasts, it completely inhibits further differentiation. AR also strongly and quickly stimulated Akt and ERK phosphorylation and c-fos and c-jun expression in an EGF receptor-dependent manner. Moreover, AR null mice displayed significantly less tibial trabecular bone than wild-type mice. Taken together, we have identified a novel growth factor that is PTH-regulated and appears to have an important role in bone metabolism.
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 53 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 58 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 16 Jan 2009, 19:17:05 EST by Maryanne Watson on behalf of Library Corporate Services