Identification of plant defence genes in canola using Arabidopsis cDNA microarrays

Schenk, P. M., Thomas-Hall, S. R., Nguyen, A. V., Manners, J. M., Kazan, K. and Spangenberg, G. (2008) Identification of plant defence genes in canola using Arabidopsis cDNA microarrays. Plant Biology, 10 5: 539-547. doi:10.1111/j.1438-8677.2008.00056.x

Author Schenk, P. M.
Thomas-Hall, S. R.
Nguyen, A. V.
Manners, J. M.
Kazan, K.
Spangenberg, G.
Title Identification of plant defence genes in canola using Arabidopsis cDNA microarrays
Journal name Plant Biology   Check publisher's open access policy
ISSN 1435-8603
Publication date 2008-09
Year available 2008
Sub-type Article (original research)
DOI 10.1111/j.1438-8677.2008.00056.x
Volume 10
Issue 5
Start page 539
End page 547
Total pages 7
Place of publication United Kingdom
Publisher Wiley-Blackwell Publishing Ltd
Collection year 2009
Language eng
Subject C1
820502 Canola
060704 Plant Pathology
Abstract We report the identification of novel defence genes in canola by using a cDNA microarray from Arabidopsis. We examined changes that occur in the abundance of transcripts corresponding to 2375 Arabidopsis expressed sequence tags (selected for defence gene identification) following inoculation of canola plants with the fungal necrotrophic leaf pathogen, Alternaria brassicicola. Microarray data obtained from this cross-hybridisation experiment were compared to expression profiles previously obtained from the equivalent Arabidopsis experiment. Homology searches using a canola expressed sequence tag database with ∼6000 unique clones led to identification of canola defence genes. Pathogen-responsive transcripts included those associated to known defence genes, reactive oxygen species metabolism, disease resistance and regulatory genes, and cell maintenance/metabolism genes. Using specific primers for quantitative real-time reverse transcriptase PCR, gene expression profiles in canola were obtained that demonstrated coordinated defence responses, including systemic responses in distal tissue and salicylic acid- and methyl jasmonate-mediated signalling against A. brassicicola.
Keyword Alternaria brassicicola
Interspecific functional genomics
Plant defence
Q-Index Code C1
Q-Index Status Confirmed Code

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Created: Thu, 15 Jan 2009, 16:07:26 EST by Gail Walter on behalf of Faculty of Science