Proteomic profiling of forskolin-induced differentiated BeWo cells: anin-vitro model of cytotrophoblast differentiation

Pottekkat Sidharthan, Neelima, Nampoothiri, Laxmipriya and Jagannadha, Rao A. (2007) Proteomic profiling of forskolin-induced differentiated BeWo cells: anin-vitro model of cytotrophoblast differentiation. Reproductive biomedicine online, 14 4 Article 2615: 477-487.


Author Pottekkat Sidharthan, Neelima
Nampoothiri, Laxmipriya
Jagannadha, Rao A.
Title Proteomic profiling of forskolin-induced differentiated BeWo cells: anin-vitro model of cytotrophoblast differentiation
Journal name Reproductive biomedicine online   Check publisher's open access policy
ISSN 1472-6483
Publication date 2007
Sub-type Article (original research)
Volume 14
Issue 4 Article 2615
Start page 477
End page 487
Total pages 11
Place of publication Cambridge
Publisher Reproductive Healthcare Ltd
Language eng
Subject 060109 Proteomics and Intermolecular Interactions (excl. Medical Proteomics)
060103 Cell Development, Proliferation and Death
Abstract Placental trophoblastic differentiation is characterized by the fusion of monolayer cytotrophoblasts into syncytiotrophoblasts. During this process of differentiation, several morphological and biochemical changes are known to occur, and this model has been employed to investigate the changes that occur at the gene and protein level during differentiation. Using the sensitive technique of proteomics [two-dimensional gel electrophoresis (2DGE)], changes in protein profile were evaluated in the control and forskolin-induced differentiated cells of trophoblastic choriocarcinoma BeWo cell line. Several proteins were differentially expressed in control and differentiated cells. Four major proteins were up-regulated as assessed by silver staining, and were further characterized as c-h-ras p 21 (phosphorylated), retinoblastoma susceptibility protein 1 and integrase interactor protein 1. These proteins are known to play an important role in growth arrest of cells, and thus may play a role in initiating the process of differentiation.
Keyword Bewo Cells
Cell cycle
Differentiation
Proliferation
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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