Inhibition of apoptosis in human tumour cells by okadaic acid

Kovacs, E. M., Lavin, M., Song, Q., Baxter, G. and Findik, D. (1992) Inhibition of apoptosis in human tumour cells by okadaic acid. Journal of Cellular Physiology, 153 3: 550-556. doi:10.1002/jcp.1041530316


Author Kovacs, E. M.
Lavin, M.
Song, Q.
Baxter, G.
Findik, D.
Title Inhibition of apoptosis in human tumour cells by okadaic acid
Journal name Journal of Cellular Physiology   Check publisher's open access policy
ISSN 1097-4652
0021-9541
Publication date 1992-12-01
Sub-type Article (original research)
DOI 10.1002/jcp.1041530316
Volume 153
Issue 3
Start page 550
End page 556
Total pages 7
Place of publication New York
Publisher Wiley
Language eng
Subject 110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics)
Abstract Gamma-radiation, tetrandrine, bistratene A, and cisplatin were all found to induce pronounced morphological changes characteristic of apoptosis and extensive DNA fragmentation in the human BM13674 cell line 8 h after treatment. Apoptosis induced in BM13674 cells by these diverse agents was markedly inhibited by 1 microM okadaic acid, a tumour promoter that inhibits protein phosphatases 1 and 2A. This compound also inhibited the appearance of apoptosis in fresh human leukaemia cells that had been exposed to gamma-radiation. The inhibition of apoptosis was confirmed using fluorescence microscopy and DNA gel electrophoresis. Dephosphorylation of a limited number of proteins was shown to be associated with apoptosis and okadaic acid prevented these dephosphorylations. Previous studies on the BM13674 cell line showed that an inhibitor of protein synthesis failed to prevent apoptosis in these cells. The present data provides further support that posttranslational modification of proteins, in particular, phosphorylation/dephosphorylation status, plays an important role in inhibition/activation of programmed cell death in different human cells after exposure to several cytotoxic agents.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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