The reference genetic linkage map for the multinational Brassica rapa genome sequencing project

Choi, Su Ryan, Teakle, Graham R., Plahal, Prikshit, Kim, Jeong Hee, Allender, Charlotte J., Beynon, Elena, Piao, Zhong Yun, Soengas, Pilar, Han, Tae Ho, King, Graham J,, Barker, Guy C., Hand, Paul, Lydiate, Derek J., Batley, Jacqueline, Edwards, David, Koo, Dal Hoe, Bang, Jae Wook, Park, Beom-Seok and Lim, Yong Pyo (2007) The reference genetic linkage map for the multinational Brassica rapa genome sequencing project. Theoretical and Applied Genetics, 115 6: 779-792. doi:10.1007/s00122-007-0608-z


Author Choi, Su Ryan
Teakle, Graham R.
Plahal, Prikshit
Kim, Jeong Hee
Allender, Charlotte J.
Beynon, Elena
Piao, Zhong Yun
Soengas, Pilar
Han, Tae Ho
King, Graham J,
Barker, Guy C.
Hand, Paul
Lydiate, Derek J.
Batley, Jacqueline
Edwards, David
Koo, Dal Hoe
Bang, Jae Wook
Park, Beom-Seok
Lim, Yong Pyo
Title The reference genetic linkage map for the multinational Brassica rapa genome sequencing project
Formatted title
The reference genetic linkage map for the multinational Brassica rapa genome sequencing project
Journal name Theoretical and Applied Genetics   Check publisher's open access policy
ISSN 0040-5752
1432-2242
Publication date 2007-10
Sub-type Article (original research)
DOI 10.1007/s00122-007-0608-z
Volume 115
Issue 6
Start page 779
End page 792
Total pages 14
Place of publication Heidelberg, Germany
Publisher Springer
Language eng
Subject 060408 Genomics
060407 Genome Structure and Regulation
Formatted abstract
We describe the construction of a reference genetic linkage map for the Brassica A genome, which will form the backbone for anchoring sequence contigs for the Multinational Brassica rapa Genome Sequencing Project. Seventy-eight doubled haploid lines derived from anther culture of the F1 of a cross between two diverse Chinese cabbage (B. rapa ssp. pekinensis) inbred lines, ‘Chiifu-401-42’ (C) and ‘Kenshin-402-43’ (K) were used to construct the map. The map comprises a total of 556 markers, including 278 AFLP, 235 SSR, 25 RAPD and 18 ESTP, STS and CAPS markers. Ten linkage groups were identified and designated as R1–R10 through alignment and orientation using SSR markers in common with existing B. napus reference linkage maps. The total length of the linkage map was 1,182 cM with an average interval of 2.83 cM between adjacent loci. The length of linkage groups ranged from 81 to 161 cM for R04 and R06, respectively. The use of 235 SSR markers allowed us to align the A-genome chromosomes of B. napus with those of B. rapa ssp. pekinensis. The development of this map is vital to the integration of genome sequence and genetic information and will enable the international research community to share resources and data for the improvement of B. rapa and other cultivated Brassica species.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Thu, 15 Jan 2009, 11:51:59 EST by Judy Dingwall on behalf of Institute for Molecular Bioscience