G{gamma}1 + G{gamma}2 != Gβ: Heterotrimeric G Protein G{gamma}-Deficient Mutants Do Not Recapitulate All Phenotypes of Gβ-Deficient Mutants

Trusov, Y., Zhang, W., Assmann, S. M. and Botella, J. R. (2008) G{gamma}1 + G{gamma}2 != Gβ: Heterotrimeric G Protein G{gamma}-Deficient Mutants Do Not Recapitulate All Phenotypes of Gβ-Deficient Mutants. Plant Physiology, 147 2: 636-649. doi:10.1104/pp.108.117655


Author Trusov, Y.
Zhang, W.
Assmann, S. M.
Botella, J. R.
Title G{gamma}1 + G{gamma}2 != Gβ: Heterotrimeric G Protein G{gamma}-Deficient Mutants Do Not Recapitulate All Phenotypes of Gβ-Deficient Mutants
Formatted title
Gγ1 + Gγ2 ≠ Gβ: Heterotrimeric G protein gγ-deficient mutants do not recapitulate all phenotypes of Gβ-deficient mutants
Journal name Plant Physiology   Check publisher's open access policy
ISSN 0032-0889
Publication date 2008-05
Year available 2008
Sub-type Critical review of research, literature review, critical commentary
DOI 10.1104/pp.108.117655
Volume 147
Issue 2
Start page 636
End page 649
Total pages 8
Place of publication United States
Publisher American Society of Plant Biologists
Collection year 2009
Language eng
Subject C1
829999 Plant Production and Plant Primary Products not elsewhere classified
060704 Plant Pathology
Formatted abstract
Heterotrimeric G proteins are signaling molecules ubiquitous among all eukaryotes. The Arabidopsis (Arabidopsis thaliana) genome contains one Gα (GPA1), one Gβ (AGB1), and two Gγ subunit (AGG1 and AGG2) genes. The Gβ requirement of a functional Gγ subunit for active signaling predicts that a mutant lacking both AGG1 and AGG2 proteins should phenotypically resemble mutants lacking AGB1 in all respects. We previously reported that Gβ- and Gγ-deficient mutants coincide during plant pathogen interaction, lateral root development, gravitropic response, and some aspects of seed germination. Here, we report a number of phenotypic discrepancies between Gβ- and Gγ-deficient mutants, including the double mutant lacking both Gγ subunits. While Gβ-deficient mutants are hypersensitive to abscisic acid inhibition of seed germination and are hyposensitive to abscisic acid inhibition of stomatal opening and guard cell inward K1 currents, none of the available Gγ-deficient mutants shows any deviation from the wild type in these responses, nor do they show the hypocotyl elongation and hook development defects that are characteristic of Gβ-deficient mutants. In addition, striking discrepancies were observed in the aerial organs of Gβ- versus Gγ-deficient mutants. In fact, none of the distinctive traits observed in Gβ-deficient mutants (such as reduced size of cotyledons, leaves, flowers, and siliques) is present in any of the Gγ single and double mutants. Despite the considerable amount of phenotypic overlap between Gβ- and Gγ-deficient mutants, confirming the tight relationship between Gβ and Gγ subunits in plants, considering the significant differences reported here, we hypothesize the existence of new and as yet unknown elements in the heterotrimeric G protein signaling complex. © 2008 American Society of Plant Biologists.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collections: 2009 Higher Education Research Data Collection
Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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