Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43

Hong, Yeon-Hee, Nishimura, Yukihiko, Hishikawa, Daisuke, Tsuzuki, Hiroaki, Miyahara, Hisae, Gotoh, Chizu, Choi, Ki-Choon, Feng, Dan Dan, Chen Chen, Lee, Hong-Gu, Katoh, Kazuo, Roh, Sang-Gun and Sasaki, Shinichi (2005) Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43. Endocrinology, 146 12: 5092-5099. doi:10.1210/en.2005-0545

Author Hong, Yeon-Hee
Nishimura, Yukihiko
Hishikawa, Daisuke
Tsuzuki, Hiroaki
Miyahara, Hisae
Gotoh, Chizu
Choi, Ki-Choon
Feng, Dan Dan
Chen Chen
Lee, Hong-Gu
Katoh, Kazuo
Roh, Sang-Gun
Sasaki, Shinichi
Title Acetate and propionate short chain fatty acids stimulate adipogenesis via GPCR43
Journal name Endocrinology   Check publisher's open access policy
ISSN 1945-7170
Publication date 2005-12
Year available 2005
Sub-type Article (original research)
DOI 10.1210/en.2005-0545
Volume 146
Issue 12
Start page 5092
End page 5099
Total pages 8
Place of publication Bethesda, USA
Publisher Endocrine Society
Language eng
Subject 110306 Endocrinology
110201 Cardiology (incl. Cardiovascular Diseases)
111201 Cancer Cell Biology
1103 Clinical Sciences
Abstract It has recently been discovered that G protein-coupled receptors (GPCR) 41 and 43 are characterized by having the short chain fatty acids acetate and propionate as their ligands. The objective of this study was to investigate the involvement of GPCR41, GPCR43, and their ligands in the process of adipogenesis. We measured the levels of GPCR41 and GPCR43 mRNA in both adipose and other tissues of the mouse. GRP43 mRNA expression was higher in four types of adipose tissue than in other tissues, whereas GPCR41 mRNA was not detected in any adipose tissues. A high level of GPCR43 expression was found in isolated adipocytes, but expression level was very low in stromal-vascular cells. Expression of GPCR43 was up-regulated in adipose tissues of mice fed a high-fat diet compared with those fed a normal-fat diet. GPCR43 mRNA could not be detected in confluent and undifferentiated 3T3-L1 adipocytes; however, the levels rose with time after the initiation of differentiation. GPCR41 expression was not detected in confluent and differentiated adipocytes. Acetate and propionate treatments increased lipids present as multiple droplets in 3T3-L1 adipocytes. Propionate significantly elevated the level of GPCR43 expression during adipose differentiation, with up-regulation of PPAR-{gamma}2. Small interfering RNA mediated a reduction of GPCR43 mRNA in 3T3-L1 cells and blocked the process of adipocyte differentiation. In addition, both acetate and propionate inhibited isoproterenol-induced lipolysis in a dose-dependent manner. We conclude that acetate and propionate short chain fatty acids may have important physiological roles in adipogenesis through GPCR43, but not through GPCR41.
Keyword Acetate
Stromal-vascular cells
Isoproterenol-induced lipolysis
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
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