Baroreceptor reflex stimulation does not induce cytomegalovirus promoter-driven transgene expression in the ventrolateral medulla in vivo

Allen, Andrew M., Dosanjh, Jaspreet, Dassanayake, Sashikala, Tan, Geraldine and Thomas, Walter G. (2006). Baroreceptor reflex stimulation does not induce cytomegalovirus promoter-driven transgene expression in the ventrolateral medulla in vivo. In: André Jean, Autonomic Neuroscience. Up-dating the Autonomic Nervous System: from Gene to Behaviour" - A Special Issue devoted to the 4th Congress of the International Society for Autonomic Neuroscience. 4th Congress of the International Society for Autonomic Neuroscience, Marseille, France, (150-155). July, 2005. doi:10.1016/j.autneu.2006.02.024

Author Allen, Andrew M.
Dosanjh, Jaspreet
Dassanayake, Sashikala
Tan, Geraldine
Thomas, Walter G.
Title of paper Baroreceptor reflex stimulation does not induce cytomegalovirus promoter-driven transgene expression in the ventrolateral medulla in vivo
Conference name 4th Congress of the International Society for Autonomic Neuroscience
Conference location Marseille, France
Conference dates July, 2005
Proceedings title Autonomic Neuroscience. Up-dating the Autonomic Nervous System: from Gene to Behaviour" - A Special Issue devoted to the 4th Congress of the International Society for Autonomic Neuroscience   Check publisher's open access policy
Journal name Autonomic Neuroscience-Basic   Check publisher's open access policy
Place of Publication Amsterdam
Publisher Elsevier B.V.
Publication Year 2006
Sub-type Fully published paper
DOI 10.1016/j.autneu.2006.02.024
ISSN 1566-0702
Editor André Jean
Volume 126– 127
Start page 150
End page 155
Total pages 6
Language eng
Abstract/Summary Abstract Adenoviruses are being employed to induce transgene expression in the central nervous system in vivo. In these studies, the cytomegalovirus (CMV) promoter is commonly employed to drive expression of the transgene because of its strong, constitutive activity in a wide range of cell types. However, using this promoter, expression in neurons is variable, with strongest expression being observed in nonneuronal cells. Indeed, even in vitro, CMV driven expression in neurons is variable. In cultured sympathetic ganglion cells it has been demonstrated that CMV-driven expression requires activation of cAMP-response element-binding protein (CREB) and that this can be induced by depolarization. In this study we tested whether depolarization might induce CMV-driven transgene expression, delivered by microinjection of an adenovirus, in the rostral ventrolateral medulla (RVLM) of rats. Prior to stimulation, transgene expression occurs in nonneuronal cells in the RVLM. Some neuronal expression was observed in neighbouring regions, in the nucleus ambiguus and in facial motor neurons. Within the RVLM, depolarization, induced by intraperitoneal administration of the ganglion blocking drug, pentolinium, did not lead to induction of transgene expression. This stimulus is known to induce expression of the immediate early gene c-fos. We conclude that either this experimental paradigm was not sufficient for activation of the CREB pathway or that possibly the virus does not gain access to the neurons of the RVLM. The adoption of specific promoters or viruses with higher neuronal transduction efficiency appears to be essential for the genetic modification of RVLM presympathetic neurons in vivo.
Subjects 060110 Receptors and Membrane Biology
060111 Signal Transduction
060199 Biochemistry and Cell Biology not elsewhere classified
060108 Protein Trafficking
060602 Animal Physiology - Cell
110201 Cardiology (incl. Cardiovascular Diseases)
110903 Central Nervous System
Keyword Rostral Ventrolateral Medulla
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