The Role of Ghrelin and Growth Hormone Secretagogues Receptor on Rat Adipogenesis

Choi, Kichoon, Roh, Sang-Gun, Hong, Yeon-Hee, Shrestha, Yogendra B, Hishikawa, Daisuke, Chen Chen, Kojima, Masayasu, Kangawa, Kenji and Sasaki, Shin-Ichi (2003) The Role of Ghrelin and Growth Hormone Secretagogues Receptor on Rat Adipogenesis. Endocrinology, 144 3: 754-759. doi:10.1210/en.2002-220783

Author Choi, Kichoon
Roh, Sang-Gun
Hong, Yeon-Hee
Shrestha, Yogendra B
Hishikawa, Daisuke
Chen Chen
Kojima, Masayasu
Kangawa, Kenji
Sasaki, Shin-Ichi
Title The Role of Ghrelin and Growth Hormone Secretagogues Receptor on Rat Adipogenesis
Journal name Endocrinology   Check publisher's open access policy
ISSN 0013-7227
Publication date 2003-03
Year available 2003
Sub-type Article (original research)
DOI 10.1210/en.2002-220783
Volume 144
Issue 3
Start page 754
End page 759
Total pages 6
Place of publication Bethesda, MA
Publisher Endocrine Society
Language eng
Subject 110306 Endocrinology
110201 Cardiology (incl. Cardiovascular Diseases)
111201 Cancer Cell Biology
Abstract Recent research progress indicates a close link between ghrelin, a natural ligand of GH secretagogues receptor (GHS-R), and both the metabolic balance and body composition. To clarify the involvement of ghrelin and GHS-R in the process of adipogenesis, we measured the expression of GHS-R and peroxisome proliferator-activated receptor {gamma} 2 (PPAR-{gamma}2) mRNA in rat adipocytes using semiquantitative RT-PCR methods. The levels of GHS-R mRNA increased by up to 4-fold in adipose tissue from epididymal and parametrial regions as the rat aged from 4–20 wk and were significantly elevated during the differentiation of preadipocytes in vitro. Ghrelin (10-8 M for 10 d) stimulated the activity of glycerol-3-phosphate dehydrogenase and the differentiation of rat preadipocytes in vitro. Ghrelin treatment also significantly increased the levels of PPAR-{gamma}2 mRNA in primary cultured rat differentiated adipocytes. In addition, isoproterenol (10-8 M, 40 min)-stimulated lipolysis was significantly reduced by simultaneous ghrelin treatment in a dose-dependent manner in vitro. In conclusion, the expression of GHS-R in rat adipocytes increases with the age and during adipogenesis. Ghrelin in vitro stimulates the differentiation of preadipocytes and antagonizes lipolysis. Ghrelin may therefore play an important role in the process of adipogenesis in rats.
Keyword Endocrinology
GH secretagogues receptor (GHS-R)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 144 times in Thomson Reuters Web of Science Article | Citations
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