Distinct Intracellular Ca2+ Response to Extracellular Adenosine Triphosphate in Pancreatic -Cells in Rats and Mice

Zhao, Yu-Feng, Xu, Ruwei, Hernandez, Maria, Zhu, Yunlong and Chen Chen (2003) Distinct Intracellular Ca2+ Response to Extracellular Adenosine Triphosphate in Pancreatic -Cells in Rats and Mice. Endocrine, 22 3: 185-192. doi:10.1385/ENDO:22:3:185


Author Zhao, Yu-Feng
Xu, Ruwei
Hernandez, Maria
Zhu, Yunlong
Chen Chen
Title Distinct Intracellular Ca2+ Response to Extracellular Adenosine Triphosphate in Pancreatic -Cells in Rats and Mice
Journal name Endocrine   Check publisher's open access policy
ISSN 0969-711X
1559-0100
Publication date 2003-12
Sub-type Article (original research)
DOI 10.1385/ENDO:22:3:185
Volume 22
Issue 3
Start page 185
End page 192
Total pages 8
Editor P. M. Conn
Place of publication Totowa, N.J.
Publisher Humana
Language eng
Subject 110306 Endocrinology
110201 Cardiology (incl. Cardiovascular Diseases)
111201 Cancer Cell Biology
Abstract Extracellular adenosine triphosphate (ATP) has distinct effects on insulin secretion from pancreatic β-cells between rats and mice. Using a confocal microscope, we compared changes between rats and mice in cytosolic free calcium concentration ([Ca2+]c) in pancreatic β-cells stimulated by extracellular ATP. Extracellular ATP (50 µM) induced calcium release from intracellular calcium stores by activating P2Y receptors in both rat and mouse β-cells. The intracellular calcium release stimulated by extracellular ATP is significantly smaller in amplitude and longer in duration in rat β-cells than in mouse. In response to extracellular ATP, rat β-cells activate store-operated calcium entry following intracellular calcium release. This response is lacking in mouse β-cells. Rat and mouse β-cells both responded to 9 mM glucose by increasing [Ca2+]c. This increase, however, was pronounced only in the rat β-cells. In 9 mM glucose, extracellular ATP induced a pro-nounced calcium release above the increased level of [Ca2+]c in rat β-cells. In mouse β-cells, however, extracellular ATP did not exhibit calcium release on top of the increased level of [Ca2+]c in 9 mM glucose. These results demonstrate distinct responses between rat and mouse β-cells to extracellular ATP under the condition of low and high glucose. Considering that extracellular ATP inhibits insulin secretion from mouse β-cells but stimulates insulin secretion from rat β-cells, we suggest that store-operated Ca2+ entry may be related to exocytosis in pancreatic rat β-cells.
Keyword Islets - insulin - β-cell - [Ca2+]
Endocrine Diseases
Endocrine glands
Endocrinology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
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Created: Tue, 13 Jan 2009, 11:28:15 EST by Ms Karen Naughton on behalf of Institute for Molecular Bioscience