Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis

Fitzgerald, Anna M., Mudge, Agnieszka M., Gleave, Andrew P. and Plummer, Kim M. (2003) Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis. Mycological Research, 107 07: 803-810.


Author Fitzgerald, Anna M.
Mudge, Agnieszka M.
Gleave, Andrew P.
Plummer, Kim M.
Title Agrobacterium and PEG-mediated transformation of the phytopathogen Venturia inaequalis
Journal name Mycological Research   Check publisher's open access policy
ISSN 1469-8102
0953-7562
Publication date 2003-07
Year available 2003
Sub-type Article (original research)
DOI 10.1017/S0953756203008086
Volume 107
Issue 07
Start page 803
End page 810
Total pages 8
Place of publication Cambridge
Publisher Elsevier
Language eng
Subject 07 Agricultural and Veterinary Sciences
0703 Crop and Pasture Production
Abstract We report the development of two new transformation systems, polyethylene glycol (PEG)-mediated transformation of protoplasts and Agrobacterium tumefaciens-mediated transformation of mycelium, for the filamentous ascomycete Venturia inaequalis. New binary vectors have been created for the latter. Although transformation was initially achieved using a PEG-mediated method, this was superseded by the A. tumefaciens-mediated approach. The advantages of the latter include: ease of the protocol, no requirement for protoplasts; higher transformation efficiency; and single-site integration. A comparison between the two transformation methods is presented.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Agriculture and Food Sciences
 
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