A molecular taxonomy for cricket paralysis virus including two new isolates from Australian populations ofDrosophila

Johnson, K. N. and Christian, P. D. (1996) A molecular taxonomy for cricket paralysis virus including two new isolates from Australian populations ofDrosophila. Archives of Virology, 141 8: 1509-1522. doi:10.1007/BF01718251


Author Johnson, K. N.
Christian, P. D.
Title A molecular taxonomy for cricket paralysis virus including two new isolates from Australian populations ofDrosophila
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
Publication date 1996-08
Year available 1996
Sub-type Article (original research)
DOI 10.1007/BF01718251
Volume 141
Issue 8
Start page 1509
End page 1522
Total pages 14
Place of publication Wien
Publisher Springer
Language eng
Subject 270000 Biological Sciences
0603 Evolutionary Biology
0605 Microbiology
Abstract Two new isolates of cricket paralysis virus, TAR and SIM, are described that were originally isolated from laboratory colonies ofDrosophila melanogaster andDrosophila simulans respectively. Using a combination of biological, serological and molecular characters it was possible to distinguish the SIM isolate from all other isolates and it is thus described as a new strain; CrPVSIM. The TAR isolate however, was indistinguishable from the CrPV reference isolate CrPVVIC/Gm/D22/Gm/D22 (Teleogryllus commodus, Victoria, Australia, 1968). The molecular characters used in the present study were obtained by combining PCR and restriction endonuclease digestion of the amplified fragments. This work demonstrates that such molecular characters, when used in combination with others, provide a powerful set of taxonomic characters for classifying CrPV isolates and strains and assessing their genetic relatedness
Keyword Biomedical and Life Sciences
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Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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