A molecular taxonomy for cricket paralysis virus including two new isolates from Australian populations ofDrosophila

Johnson, K. N. and Christian, P. D. (1996) A molecular taxonomy for cricket paralysis virus including two new isolates from Australian populations ofDrosophila. Archives of Virology, 141 8: 1509-1522.

Author Johnson, K. N.
Christian, P. D.
Title A molecular taxonomy for cricket paralysis virus including two new isolates from Australian populations ofDrosophila
Journal name Archives of Virology   Check publisher's open access policy
ISSN 0304-8608
Publication date 1996-08
Year available 1996
Sub-type Article (original research)
DOI 10.1007/BF01718251
Volume 141
Issue 8
Start page 1509
End page 1522
Total pages 14
Place of publication Wien
Publisher Springer
Language eng
Subject 270000 Biological Sciences
0603 Evolutionary Biology
0605 Microbiology
Abstract Two new isolates of cricket paralysis virus, TAR and SIM, are described that were originally isolated from laboratory colonies ofDrosophila melanogaster andDrosophila simulans respectively. Using a combination of biological, serological and molecular characters it was possible to distinguish the SIM isolate from all other isolates and it is thus described as a new strain; CrPVSIM. The TAR isolate however, was indistinguishable from the CrPV reference isolate CrPVVIC/Gm/D22/Gm/D22 (Teleogryllus commodus, Victoria, Australia, 1968). The molecular characters used in the present study were obtained by combining PCR and restriction endonuclease digestion of the amplified fragments. This work demonstrates that such molecular characters, when used in combination with others, provide a powerful set of taxonomic characters for classifying CrPV isolates and strains and assessing their genetic relatedness
Keyword Biomedical and Life Sciences
References 1. Brun G, Plus N (1980) The viruses ofDrosophila. In: Ashburner M, Wright TRF (eds) The biology and genetics ofDrosophila, Vol 3a. Academic Press, New York, pp 625–702 2. Chen Q, McIntosh AH, Ignoffo CM (1983) Establishment of a new cell line from the pupae ofPlutella xylostella J Centr China Teach Coll 3: 99–107 3. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159 4. Christian PD, Scotti PD (1994) A suggested taxonomy and nomenclature for Cricket paralysis virus andDrosophila C virus complex. J Invertebr Pathol 63: 157–162 5. King LA, Pullin JSK, Stanway G, Almond JW, Moore NF (1987) Cloning of the genome of an insect picornavirus using a cDNA: RNA hybrid method: sequence of the 3prime end. Virus Res 6: 331–344 6. Koonin EV, Gorbalenya AE (1992) An insect picornavirus may have a genome organisation similar to that of caliciviruses. FEBS Lett 297: 81–86 7. McIntosh AH, Maramorosch K, Rechtoris C (1973) Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium. In vitro 8: 375–378 8. McIntosh AH, Rechtoris C (1974) Insect cells: colony formation and cloning in agar medium. In vitro 10: 1–5 9. McIntosh AH, Ignoffo CM (1981) Replication and infectivity of the single-embedded nuclear polyhedrosis virus,Baculovirus heliothis, in homologous cell lines. J Invertebr Pathol 37: 258–264 10. McIntosh AH, Ignoffo CM (1989) Replication ofAutographa californica nuclear polyhedrosis virus in five lepidopteran cell lines. J Invertebr Pathol 54: 97–102 11. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory Press, New York 12. Manousis T, Moore NF (1987) Cricket paralysis virus, a potential control agent for the olive fruit fly,Dacus oleae Gmal. Appl Environ Microbiol 53: 142–148 13. Mansi W (1958) Slide gel-diffusion precipitation test. Nature 181: 1289 14. Nei M, Li WH (1979) Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc Natl Acad Sci USA 76: 5269–5273 15. Nei M, Miller JC (1990) A simple method for estimating average number of nucleotide substitutions within and between populations from restriction data. Genetics 125: 873–879 16. Peleg J (1968) Growth of arboviruses in monolayers from subcultured mosquito embryo cells. Virology 35: 317–319 17. Plus N, Scotti PD (1984) The biological properties of eight different isolates of cricket paralysis virus. Ann Virol Inst Pasteur 135E: 257–268 18. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic tress. Mol Biol Evol 4: 406–425 19. Schneider I (1972) Cell lines derived from late embryonic stages ofDrosophila melanogaster. J Embryol Exp Morph 27: 353–365 20. Scotti PD (1976) Cricket paralysis virus replicates in culturedDrosophila cells. Intervirology 6: 333–342 21. Scotti PD (1977) End-point dilution and plaque assay methods for titration of cricket paralysis virus in culturedDrosophila cells. J Gen Virol 35: 393–396 22. Scotti PD, Longworth JF, Plus N, Croizier G, Reinganum C (1981) The biology and ecology of strains of an insect small RNA virus complex. Adv Virus Res 26: 117–143 23. Scotti PD, Wigley P (1982) Factors affecting the use of immunoosmophoresis for the detection of two insect riboviruses. J Virol Methods 4: 129–137 24. Singh KRP (1967) Cell cultures derived from larvae ofAedes albopictus Skuse andAedes aegypti (L.) Curr Sci 36: 506–508 25. Sneath PHA, Sokal RR (1973) Numerical taxonomy. Freeman, San Francisco 26. Summers MD, Smith GG (1987) A manual of methods for baculovirus vectors and insect cell culture procedures. Texas Agric Exp Stat Bull No. 1555 27. Vaughn JL, Goodwin RH, Tompkins GJ, McCawley P (1977) Establishment of two cell lines from the insectSpodoptera frugiperda (Lepidoptera: Noctuidae). In vitro 13: 213–217
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

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