Localization of plasma membrane and secretory calcium pumps in the mammary gland

Faddy, Helen M., Smart, Chanel E., Xu, Ren, Lee, Genee Y., Kenny, Paraic A., Feng, Mingye, Rao, Rajini, Brown, Melissa A., Bissell, Mina J., Roberts-Thomson, Sarah J. and Monteith, Gregory R. (2008) Localization of plasma membrane and secretory calcium pumps in the mammary gland. Biochemical and Biophysical Research Communications, 369 3: 977-981. doi:10.1016/j.bbrc.2008.03.003

Author Faddy, Helen M.
Smart, Chanel E.
Xu, Ren
Lee, Genee Y.
Kenny, Paraic A.
Feng, Mingye
Rao, Rajini
Brown, Melissa A.
Bissell, Mina J.
Roberts-Thomson, Sarah J.
Monteith, Gregory R.
Title Localization of plasma membrane and secretory calcium pumps in the mammary gland
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
Publication date 2008-05-09
Year available 2008
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2008.03.003
Volume 369
Issue 3
Start page 977
End page 981
Total pages 5
Editor W. Baumeister
Place of publication New York, N.Y. U.S.A.
Publisher Academic Press
Collection year 2009
Language eng
Subject 920102 Cancer and Related Disorders
060199 Biochemistry and Cell Biology not elsewhere classified
Abstract Until recently the mechanism for the enrichment of milk with calcium was thought to be almost entirely via the secretory pathway. However, recent studies suggest that a plasma membrane calcium ATPase, PMCA2, is the primary mechanism for calcium transport into milk, highlighting a major role for apical calcium transport. We compared the expression of the recently identified secretory calcium ATPase, SPCA2, and SPCA1, in the mouse mammary gland during development. SPCA2 levels increased over 35-fold during lactation with expression localized to luminal secretory cells, while SPCA1 increased only a modest 2-fold and was expressed throughout the cells of the mammary gland. We also observed major differences in the localization of PMCA2 and PMCA1. Our studies highlight the likely specific roles of PMCA2 and SPCA2 in lactation and indicate that calcium transport into milk is a complex interplay between apical and secretory pathways.
Keyword Calcium
Mammary gland
3D mammary culture
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Pharmacy Publications
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Citation counts: TR Web of Science Citation Count  Cited 39 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 10 Dec 2008, 14:10:25 EST by Elizabeth Pyke on behalf of School of Pharmacy