Drosophila melanogaster mounts a unique immune response to the Rhabdovirus Sigma virus

Tsai, C.W., McGraw, E. A., Ammar, E.-D., Dietzgen, R.G. and Hogenhout, S.A. (2008) Drosophila melanogaster mounts a unique immune response to the Rhabdovirus Sigma virus. Applied and Environmental Microbiology, 74 10: 3251-3256. doi:10.1128/AEM.02248-07

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Author Tsai, C.W.
McGraw, E. A.
Ammar, E.-D.
Dietzgen, R.G.
Hogenhout, S.A.
Title Drosophila melanogaster mounts a unique immune response to the Rhabdovirus Sigma virus
Formatted title
Drosophila melanogaster mounts a unique immune response to the Rhabdovirus Sigma virus
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2008-05
Sub-type Article (original research)
DOI 10.1128/AEM.02248-07
Open Access Status File (Publisher version)
Volume 74
Issue 10
Start page 3251
End page 3256
Total pages 6
Place of publication United States
Publisher American Society for Microbiology
Collection year 2009
Language eng
Subject C1
970106 Expanding Knowledge in the Biological Sciences
060307 Host-Parasite Interactions
0605 Microbiology
Abstract Rhabdoviruses are important pathogens of humans, livestock, and plants that are often vectored by insects. Rhabdovirus particles have a characteristic bullet shape with a lipid envelope and surface-exposed transmembrane glycoproteins. Sigma virus (SIGMAV) is a member of the Rhabdoviridae and is a naturally occurring disease agent of Drosophila melanogaster. The infection is maintained in Drosophila populations through vertical transmission via germ cells. We report here the nature of the Drosophila innate immune response to SIGMAV infection as revealed by quantitative reverse transcription-PCR analysis of differentially expressed genes identified by microarray analysis. We have also compared and contrasted the immune response of the host with respect to two nonenveloped viruses, Drosophila C virus (DCV) and Drosophila X virus (DXV). We determined that SIGMAV infection upregulates expression of the peptidoglycan receptor protein genes PGRP-SB1 and PGRP-SD and the antimicrobial peptide (AMP) genes Diptericin-A, Attacin-A, Attacin-B, Cecropin-A1, and Drosocin. SIGMAV infection did not induce PGRP-SA and the AMP genes Drosomycin-B, Metchnikowin, and Defensin that are upregulated in DCV and/or DXV infections. Expression levels of the Toll and Imd signaling cascade genes are not significantly altered by SIGMAV infection. These results highlight shared and unique aspects of the Drosophila immune response to the three viruses and may shed light on the nature of the interaction with the host and the evolution of these associations.
Formatted abstract
Rhabdoviruses are important pathogens of humans, livestock, and plants that are often vectored by insects. Rhabdovirus particles have a characteristic bullet shape with a lipid envelope and surface-exposed transmembrane glycoproteins. Sigma virus (SIGMAV) is a member of the Rhabdoviridae and is a naturally occurring disease agent of Drosophila melanogaster. The infection is maintained in Drosophila populations through vertical transmission via germ cells. We report here the nature of the Drosophila innate immune response to SIGMAV infection as revealed by quantitative reverse transcription-PCR analysis of differentially expressed genes identified by microarray analysis. We have also compared and contrasted the immune response of the host with respect to two nonenveloped viruses, Drosophila C virus (DCV) and Drosophila X virus (DXV). We determined that SIGMAV infection upregulates expression of the peptidoglycan receptor protein genes PGRP-SB1 and PGRP-SD and the antimicrobial peptide (AMP) genes Diptericin-A, Attacin-A, Attacin-B, Cecropin-A1, and Drosocin. SIGMAV infection did not induce PGRP-SA and the AMP genes Drosomycin-B, Metchnikowin, and Defensin that are upregulated in DCV and/or DXV infections. Expression levels of the Toll and Imd signaling cascade genes are not significantly altered by SIGMAV infection. These results highlight shared and unique aspects of the Drosophila immune response to the three viruses and may shed light on the nature of the interaction with the host and the evolution of these associations.
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biological Sciences Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 27 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 21 Nov 2008, 17:31:04 EST by Gail Walter on behalf of School of Biological Sciences