An insect virus-encoded microRNA regulates viral replication

Hussain, Mazhar, Taft, Ryan J. and Asgari, Sassan (2008) An insect virus-encoded microRNA regulates viral replication. Journal of Virology, 82 18: 9164-9170. doi:10.1128/JVI.01109-08

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Author Hussain, Mazhar
Taft, Ryan J.
Asgari, Sassan
Title An insect virus-encoded microRNA regulates viral replication
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 2008-09-01
Year available 2008
Sub-type Article (original research)
DOI 10.1128/JVI.01109-08
Open Access Status File (Publisher version)
Volume 82
Issue 18
Start page 9164
End page 9170
Total pages 7
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2009
Language eng
Subject 970106 Expanding Knowledge in the Biological Sciences
060506 Virology
0605 Microbiology
Formatted abstract
MicroRNAs (miRNAs) are small (~22 nucleotides) noncoding RNAs which play an essential role in gene regulation and affect a wide range of processes, including development, differentiation, and oncogenesis. Here we report the identification of the first miRNA from an insect virus, derived from the major capsid protein (MCP) gene in Heliothis virescens ascovirus (HvAV) (HvAV-miR-1). Although MCP was abundantly expressed at all time points 24 h after infection, HvAV-miR-1 expression was strictly regulated and specifically detected from 96 h postinfection. HvAV-miR-1 expression coincided with a marked reduction of the expression of HvAV DNA polymerase I, which is a predicted target. Ectopic expression of full-length and truncated versions of MCP retaining the miRNA sequence significantly reduced DNA polymerase I transcript levels and inhibited viral replication. Our results indicate that HvAV-miR-1 directs transcriptional degradation of DNA polymerase I and regulates HvAV replication. These findings are congruent with recent reports that miR-BART-2 regulates Epstein-Barr virus DNA polymerase expression and suggest that virus-encoded miRNA regulation of virus replication may be a general phenomenon.
Keyword Heliothis-Virescens Ascovirus
Posttranscriptional Regulation
EST Database
Web Server
Q-Index Code C1
Q-Index Status Confirmed Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2009 Higher Education Research Data Collection
School of Biological Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 70 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 70 times in Scopus Article | Citations
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Created: Fri, 14 Nov 2008, 13:48:33 EST by Gail Walter on behalf of School of Biological Sciences