Characterising the macroporosity Of polymeric matrices designed for protein delivery and tissue engineering

Wang, Yiwei, Chang, Hsin-I, Wertheim, David F., Jones, Allan S., Jackson, Chris and Coombes, Allan G.A. (2007). Characterising the macroporosity Of polymeric matrices designed for protein delivery and tissue engineering. In: D. Traini and J. Hanrahan, Proceedings the Annual Conference Of The Australasian Pharmaceutical Science Association. 2007 Annual Confernece Medicines: Design to Delivery, Sydney, Australia, (). 8 - 11 December 2007.

Author Wang, Yiwei
Chang, Hsin-I
Wertheim, David F.
Jones, Allan S.
Jackson, Chris
Coombes, Allan G.A.
Title of paper Characterising the macroporosity Of polymeric matrices designed for protein delivery and tissue engineering
Conference name 2007 Annual Confernece Medicines: Design to Delivery
Conference location Sydney, Australia
Conference dates 8 - 11 December 2007
Proceedings title Proceedings the Annual Conference Of The Australasian Pharmaceutical Science Association   Check publisher's open access policy
Publication Year 2007
Year available 2007
Sub-type Fully published paper
ISBN 9780646484587
ISSN 0142-9612
1878-5905
Editor D. Traini
J. Hanrahan
Collection year 2008
Language eng
Abstract/Summary Microporous, biocomposite matrices comprising a continuous phase of poly(ε-caprolactone) (PCL) and a dispersed phase of lactose or gelatin particles with defined size range (45–90, 90–125 and 125–250 μm) were produced by precipitation casting from solutions of PCL in acetone. Scanning electron microscopy (SEM) analysis revealed a characteristic surface morphology of particulates interspersed amongst crystalline lamellae of the polymer phase. Rapid release of around 80% of the lactose content occurred in PBS at 37 °C in 3 days, whereas biocomposites containing gelatin particles of size range 90–125 and 125–250 μm, respectively, displayed gradual and highly efficient release of around 90% of the protein phase over 21 days. A highly porous structure was obtained on extraction of the water-soluble phase. Micro-computed tomography (Micro-CT) and image analysis enabled 3-D visualisation and quantification of the internal pore size distribution. A maximum fractional pore area of 10.5% was estimated for gelatin-loaded matrices. Micro-CT analysis confirmed the presence of an extensive system of macropores, sufficiently connected to permit protein diffusion, but an absence of high volume, inter-pore channels. Thus tissue integration would be confined to the matrix surface initially if the designs investigated were used as tissue-engineering scaffolds, with the core potentially providing a depot system for controlled delivery of growth factors.
Subjects 1115 Pharmacology and Pharmaceutical Sciences
Q-Index Code EX
Q-Index Status Provisional Code
Institutional Status Non-UQ

 
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Created: Wed, 03 Sep 2008, 11:26:32 EST by Linda Mclean on behalf of School of Pharmacy