The relationship between erythrocyte transketolase activity and the 'TPP effect' in Wernicke's encephalopathy and other thiamine deficiency states

Nixon, Peter F., Price, John, Norman-Hicks, Maureen, Wiliams, Gail M. and Kerr, Ray A (1990) The relationship between erythrocyte transketolase activity and the 'TPP effect' in Wernicke's encephalopathy and other thiamine deficiency states. Clinica Chimica Acta, 192 2: 89-98. doi:10.1016/0009-8981(90)90072-Z


Author Nixon, Peter F.
Price, John
Norman-Hicks, Maureen
Wiliams, Gail M.
Kerr, Ray A
Title The relationship between erythrocyte transketolase activity and the 'TPP effect' in Wernicke's encephalopathy and other thiamine deficiency states
Journal name Clinica Chimica Acta   Check publisher's open access policy
ISSN 0009-8981
1873-3492
Publication date 1990-11
Sub-type Article (original research)
DOI 10.1016/0009-8981(90)90072-Z
Volume 192
Issue 2
Start page 89
End page 98
Total pages 10
Place of publication Amsterdam, The Netherlands
Publisher Elsevier
Language eng
Subject 1103 Clinical Sciences
Formatted abstract
Patients (n = 104) were judged to be thiamine deficient by the criteria of erythrocyte transketolase activity (ETK) less than 0.6 U/g of hemoglobin, or greater than 17% increase in this activity on addition of thiamine pyrophosphate in vitro (TPP effect). ETK activated by TPP in vitro (AETK) was related to ETK by a linear regression of slope ≥1, implying that transketolase apoenzyme (apoTK) was constant or decreased as ETK decreased. For most patient groups the value of apoTK was 0.1 U/g and the slope 1.033 to 1.050. In the subgroup of non-vomiting drinkers with Wernicke's encephalopathy (WE), the slope of the linear regression of AETK on ETK was 1.21, so that apoTK decreased as ETK decreased. Comparison of these data is consistent with a difference in the TK of WE drinkers from that of others. Generally, any variation of TPP effect was due only to variation of ETK. We recommend measurement of ETK, without TPP effect, for the assessment of thiamine nutrition.
Keyword erythrocyte
thiamine deficiency
thiamine diphosphate
TPP effect
transketolase
Wernicke's encephalopathy
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Public Health Publications
 
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Created: Thu, 28 Aug 2008, 16:15:23 EST