The Fusarium mycotoxin deoxynivalenol elicits hydrogen peroxide production, programmed cell death and defence responses in wheat

Desmond, Olivia J., Manners, John M., Stephens, Amber E., Maclean, Donald J., Schenk, Peer M., Gardiner, Donald M., Munn, Alan L. and Kazan, Kemal (2008) The Fusarium mycotoxin deoxynivalenol elicits hydrogen peroxide production, programmed cell death and defence responses in wheat. Molecular Plant Pathology, 9 4: 435-445. doi:10.1111/J.1364-3703.2008.00475.X


Author Desmond, Olivia J.
Manners, John M.
Stephens, Amber E.
Maclean, Donald J.
Schenk, Peer M.
Gardiner, Donald M.
Munn, Alan L.
Kazan, Kemal
Title The Fusarium mycotoxin deoxynivalenol elicits hydrogen peroxide production, programmed cell death and defence responses in wheat
Journal name Molecular Plant Pathology   Check publisher's open access policy
ISSN 1464-6722
Publication date 2008-07
Sub-type Article (original research)
DOI 10.1111/J.1364-3703.2008.00475.X
Open Access Status
Volume 9
Issue 4
Start page 435
End page 445
Total pages 11
Place of publication England
Publisher Blackwell Science in collaboration with the British Society of Plant Pathology
Collection year 2009
Language eng
Subject C1
03 Chemical Sciences
0607 Plant Biology
060702 Plant Cell and Molecular Biology
Abstract Fusarium species infect cereal crops worldwide and cause the important diseases Fusarium head blight and crown rot in wheat. Fusarium pathogens reduce yield and some species also produce trichothecene mycotoxins, such as deoxynivalenol (DON), during infection. These toxins play roles in pathogenesis on wheat and have serious health effects if present in grain consumed by humans or animals. In the present study, the response of wheat tissue to DON has been investigated. Infusion of wheat leaves with DON induced hydrogen peroxide production within 6 h followed by cell death within 24 h that was accompanied by DNA laddering, a hallmark of programmed cell death. In addition, real-time PCR analysis revealed that DON treatment rapidly induced transcription of a number of defence genes in a concentration-dependent manner. Co-treatment with DON and the antioxidant ascorbic acid reduced these responses, suggesting their induction may be at least partially mediated by reactive oxygen species (ROS), commonly known to be signalling molecules in plants. Wheat defence genes were more highly expressed in wheat stems inoculated with a DON-producing fungal strain than those inoculated with a DON-non-producing mutant, but only at a late stage of infection. Taken together, the results are consistent with a model in which DON production during infection of wheat induces ROS, which on the one hand may stimulate programmed host cell death assisting necrotrophic fungal growth, whereas, on the other hand, the ROS may contribute to the induction of antimicrobial host defences.
Keyword Induced Gene-expression
Arabidopsis-thaliana
Trichodiene Synthase
Head Blight
Oxidative Burst
Graminearum
Trichothecenes
Resistance
Pseudograminearum
Infection
Q-Index Code C1
Q-Index Status Confirmed Code

 
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Created: Tue, 29 Jul 2008, 16:17:46 EST