Remote control of neuronal activity with a light-gated glutamate receptor

Szobota, Stephanie, Gorostiza, Pau, Del Bene, Filippo, Wyart, Claire, Fortin, Doris L., Kolstad, Kathleen D., Tulyathan, Orapim, Volgraf, Matthew, Numano, Rika, Aaron, Holly L., Scott, Ethan K., Kramer, Richard H., Flannery, John, Baier, Herwig, Trauner, Dirk and Isacoff, Ehud Y. (2007) Remote control of neuronal activity with a light-gated glutamate receptor. Neuron, 54 4: 535-545. doi:10.1016/j.neuron.2007.05.010


Author Szobota, Stephanie
Gorostiza, Pau
Del Bene, Filippo
Wyart, Claire
Fortin, Doris L.
Kolstad, Kathleen D.
Tulyathan, Orapim
Volgraf, Matthew
Numano, Rika
Aaron, Holly L.
Scott, Ethan K.
Kramer, Richard H.
Flannery, John
Baier, Herwig
Trauner, Dirk
Isacoff, Ehud Y.
Title Remote control of neuronal activity with a light-gated glutamate receptor
Journal name Neuron   Check publisher's open access policy
ISSN 0896-6273
1097-4199
Publication date 2007-05-24
Sub-type Article (original research)
DOI 10.1016/j.neuron.2007.05.010
Volume 54
Issue 4
Start page 535
End page 545
Total pages 11
Place of publication Cambridge, MA, United States
Publisher Cell Press
Language eng
Abstract The ability to stimulate select neurons in isolated tissue and in living animals is important for investigating their role in circuits and behavior. We show that the engineered light-gated ionotropic glutamate receptor (LiGluR), when introduced into neurons, enables remote control of their activity. Trains of action potentials are optimally evoked and extinguished by 380 nm and 500 nm light, respectively, while intermediate wavelengths provide graded control over the amplitude of depolarization. Light pulses of 1–5 ms in duration at not, vert, similar380 nm trigger precisely timed action potentials and EPSP-like responses or can evoke sustained depolarizations that persist for minutes in the dark until extinguished by a short pulse of not, vert, similar500 nm light. When introduced into sensory neurons in zebrafish larvae, activation of LiGluR reversibly blocks the escape response to touch. Our studies show that LiGluR provides robust control over neuronal activity, enabling the dissection and manipulation of neural circuitry in vivo.
Keyword Sysneuro
Cellbio
Molneuro
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biomedical Sciences Publications
 
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Created: Fri, 18 Jul 2008, 11:29:51 EST by Laura McTaggart on behalf of School of Biomedical Sciences