Can liquid chromatography-tandem mass spectrometry solve the problem of aldosterone measurement?

Taylor, P., Carlton, L., Franklin, M., Cooper, D., Gordon, R.D. and Stowasser, M. (2007). Can liquid chromatography-tandem mass spectrometry solve the problem of aldosterone measurement?. In: Hypertension. 28th annual Scientific Meeting of the High Blood Pressure Research Council of Australia, Brisbane, (1478-1478). 7-8 December, 2006. doi:10.1161/HYPERTENSIONAHA.107.009421


Author Taylor, P.
Carlton, L.
Franklin, M.
Cooper, D.
Gordon, R.D.
Stowasser, M.
Title of paper Can liquid chromatography-tandem mass spectrometry solve the problem of aldosterone measurement?
Conference name 28th annual Scientific Meeting of the High Blood Pressure Research Council of Australia
Conference location Brisbane
Conference dates 7-8 December, 2006
Proceedings title Hypertension   Check publisher's open access policy
Journal name Hypertension   Check publisher's open access policy
Place of Publication Philadelphia
Publisher Lippincott Williams & Wilkins
Publication Year 2007
DOI 10.1161/HYPERTENSIONAHA.107.009421
ISSN 0194-911X
1524-4563
Volume 49
Issue 6
Start page 1478
End page 1478
Total pages 1
Language eng
Formatted Abstract/Summary
Detection of endocrine causes of hypertension such as primary aldosteronism (PAL) offers the
opportunity to cure or achieve improved control through specific treatment. Reliable measurement
of aldosterone is an essential requirement for accurate detection of PAL. Currently,
aldosterone measurement is based on radioimmunoassay or chemiluminescence techniques.
However, problems exist with the specificity of these approaches. Hence, there is an urgent
need to develop a highly reliable method of measuring aldosterone. Liquid chromatographyelectrospray-
tandem mass spectrometry (LC-MS) is a new technology that is being adopted for
use in the clinical laboratory. The aim of this study was to investigate the feasibility of using
LC-MS to measure aldosterone in human plasma. Samples were prepared and analysed using
a Symbiosis on-line sample preparation/LC system coupled to a Quattro Premier tandem-mass
spectrometer. For on-line sample preparation, an Oasis HLB extraction cartridge (10 x 1 mm,
30 m) was preconditioned with acetonitrile (1 mL) followed by water (1 mL), and the loaded
cartridge washed sequentially with 2% ammonium hydroxide (1 mL), water (1 mL), 5%
acetonitrile in 2% formic acid (1 mL) and water (2 mL). The sample was chromatographed
using gradient elution on a Sunfire C18 (50 x 2.1 mm, 3.5 m) analytical column. The mobile
phase consisted of acetonitrile and water. Retention time for aldosterone was 3.9 min with an
overall analysis time of 7 min. Aldosterone was detected in negative electrospray ionisation
mode using selected reaction monitoring (m/z 359.23331.4). Using water based standards
(250 L) the method was found to be linear over the range 18.6 to 3700 pg/mL (r2 0.996).
However, a non-linear response and increased variability, possibly due to matrix effects, was
found when increasing the plasma sample volume from 100 to 250 L. Thus all plasma
experiments were performed using 100 L. This resulted in a linear range for the plasma assay
of 58 to 925 pg/mL (r2 0.995). While some refinement is required to improve lower limits
of quantification for plasma, LC-MS using on-line (Symbiosis) sample preparation has the
potential to provide highly accurate measurement of aldosterone and thus facilitate improved
diagnosis and management of PAL.
Subjects 321004 Endocrinology
730105 Endocrine organs and diseases (incl. diabetes)
EX
Keyword hypertension
high blood pressure
Q-Index Code EX

 
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Created: Thu, 03 Jul 2008, 15:23:56 EST by Anne Draper on behalf of Medicine - Princess Alexandra Hospital