Spatial aspects of Ca2+ signalling in pancreatic acinar cells

Thorn, Peter (1993) Spatial aspects of Ca2+ signalling in pancreatic acinar cells. Journal of Experimental Biology, 184 1: 129-144.

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Author Thorn, Peter
Title Spatial aspects of Ca2+ signalling in pancreatic acinar cells
Journal name Journal of Experimental Biology   Check publisher's open access policy
ISSN 0022-0949
Publication date 1993-11
Sub-type Critical review of research, literature review, critical commentary
Open Access Status File (Publisher version)
Volume 184
Issue 1
Start page 129
End page 144
Total pages 16
Place of publication Cambridge, England
Publisher Company of Biologists
Language eng
Subject 320300 Medical Biochemistry and Clinical Chemistry
Abstract Secretory cells do not only respond to an agonist with a simple rise in [Ca2+]i. It is now clear that complex patterns of [Ca2+]i elevation in terms of space and time are observed in many cell types and that these patterns may be a cellular mechanism for the regulation of different responses. Ca2+ signalling in exocrine cells of the pancreas promotes the secretion of digestive enzymes and fluid. It has been shown that at high concentrations of agonist (acetylcholine or cholecystokinin) the [Ca2+]i response is initiated in the secretory pole of the cell before spreading across the whole cell. This site of initiation of the [Ca2+]i elevation is in the region where exocytotic release of enzymes occurs and is also the site of a Ca(2+)-dependent chloride channel thought to be crucially important for fluid secretion. Lower concentrations of agonist elicit [Ca2+]i oscillations with complex repetitive patterns characteristic of each agonist. At physiological agonist concentrations, we have recently described repetitive short-lasting Ca2+ spikes that are spatially restricted to the secretory pole of the cell. In addition to these spikes, cholecystokinin also promotes slow transient Ca2+ rises that result in a global rise in Ca2+. The inositol trisphosphate (InsP3) receptor plays a crucial role in all of these various agonist responses, most of which can be reproduced by the infusion of InsP3 into the cell. The high InsP3-sensitivity of the secretory pole is postulated to be due to a localization of high-affinity InsP3 receptors. We speculate that in response to cholecystokinin the short-lasting spikes elicit exocytosis from a small 'available pool' of vesicles and that the broader oscillations induce both exocytosis and cell changes that involve movement of vesicles into this 'available pool'.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Critical review of research, literature review, critical commentary
Collection: School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 14 times in Thomson Reuters Web of Science Article | Citations
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Created: Thu, 19 Jun 2008, 11:38:42 EST by Laura McTaggart on behalf of School of Biomedical Sciences