A bimodal pattern of InsP(3)-evoked elementary Ca(2+) signals in pancreatic acinar cells

Fogarty, K. E., Kidd, J. F., Tuft, R. A. and Thorn, P. (2000) A bimodal pattern of InsP(3)-evoked elementary Ca(2+) signals in pancreatic acinar cells. Biophysical Journal, 78 5: 2298-2306.


Author Fogarty, K. E.
Kidd, J. F.
Tuft, R. A.
Thorn, P.
Title A bimodal pattern of InsP(3)-evoked elementary Ca(2+) signals in pancreatic acinar cells
Journal name Biophysical Journal   Check publisher's open access policy
ISSN 0006-3495
Publication date 2000-05
Sub-type Article (original research)
Volume 78
Issue 5
Start page 2298
End page 2306
Total pages 9
Place of publication Bethesda, MD.
Publisher Biophysical Society
Language eng
Subject 250599 Macromolecular Chemistry not elsewhere classified
270399 Microbiology not elsewhere classified
Abstract InsP(3)-evoked elementary Ca(2+) release events have been postulated to play a role in providing the building blocks of larger Ca(2+) signals. In pancreatic acinar cells, low concentrations of acetylcholine or the injection of low concentrations of InsP(3) elicit a train of spatially localized Ca(2+) spikes. In this study we have quantified these responses and compared the Ca(2+) signals to the elementary events shown in Xenopus oocytes. The results demonstrate, at the same concentrations of InsP(3), Ca(2+) signals consisting of one population of small transient Ca(2+) release events and a second distinct population of larger Ca(2+) spikes. The signal mass amplitudes of both types of events are within the range of amplitudes for the elementary events in Xenopus oocytes. However, the bimodal Ca(2+) distribution of Ca(2+) responses we observe is not consistent with the continuum of event sizes seen in Xenopus. We conclude that the two types of InsP(3)-dependent events in acinar cells are both elementary Ca(2+) signals, which are independent of one another. Our data indicate a complexity to the organization of the Ca(2+) release apparatus in acinar cells, which might result from the presence of multiple InsP(3) receptor isoforms, and is likely to be important in the physiology of these cells.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Thu, 19 Jun 2008, 09:02:46 EST by Laura McTaggart on behalf of School of Biomedical Sciences