Carbachol-stimulated chloride secretion in mouse colon: Evidence of a role for autocrine prostaglandin E2 release

Carew, Mark A. and Thorn, Peter (2000) Carbachol-stimulated chloride secretion in mouse colon: Evidence of a role for autocrine prostaglandin E2 release. Experimental Physiology, 85 1: 67-72. doi:10.1111/j.1469-445X.2000.01947.x


Author Carew, Mark A.
Thorn, Peter
Title Carbachol-stimulated chloride secretion in mouse colon: Evidence of a role for autocrine prostaglandin E2 release
Journal name Experimental Physiology   Check publisher's open access policy
ISSN 0958-0670
Publication date 2000-01
Sub-type Article (original research)
DOI 10.1111/j.1469-445X.2000.01947.x
Volume 85
Issue 1
Start page 67
End page 72
Total pages 6
Place of publication Oxford, United Kingdom
Publisher Blackwell
Language eng
Subject 270602 Animal Physiology - Cell
Abstract We used the short-circuit current technique to investigate the possible facilitatory role of epithelium-derived prostaglandin E2 (PGE2) release on Cl− secretion in the mouse colon. Carbachol- (CCh)-stimulated Cl− secretion was reduced by pretreatment with either indomethacin (10 μM), or TTX (1 μM), and when added together, these inhibitors revealed net CCh-stimulated K+ secretion. CCh-stimulated Cl− secretion was partially restored to TTX/indomethacin-treated colons by addition of a subsecretory concentration of PGE2 (1 nM). In acutely isolated, unstimulated crypt cells, we measured PGE2 release at a similar level. We conclude that autocrine release of PGs from epithelial cells is sufficient to support the CCh-induced Cl− secretory response and is a likely co-factor in this response.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Thu, 19 Jun 2008, 08:51:48 EST by Laura McTaggart on behalf of School of Biomedical Sciences