SR2 elements, non-long terminal repeat retrotransposons of the RTE-1 lineage from the human blood fluke Schistosoma mansoni

Drew, Alexander C., Minchella, Dennis J., King, Lynette T., Rollinson, David and Brindley, Paul J. (1999) SR2 elements, non-long terminal repeat retrotransposons of the RTE-1 lineage from the human blood fluke Schistosoma mansoni. Molecular Biology and Evolution, 16 9: 1256-1269.


Author Drew, Alexander C.
Minchella, Dennis J.
King, Lynette T.
Rollinson, David
Brindley, Paul J.
Title SR2 elements, non-long terminal repeat retrotransposons of the RTE-1 lineage from the human blood fluke Schistosoma mansoni
Formatted title
SR2 elements, non-long terminal repeat retrotransposons of the RTE-1 lineage from the human blood fluke Schistosoma mansoni
Journal name Molecular Biology and Evolution   Check publisher's open access policy
ISSN 0737-4038
1537-1719
Publication date 1999-09
Sub-type Article (original research)
Volume 16
Issue 9
Start page 1256
End page 1269
Total pages 14
Place of publication Lawrence, KS, U.S.A.
Publisher Society for Molecular Biology
Collection year 1999
Language eng
Subject C1
320405 Medical Parasitology
730101 Infectious diseases
0604 Genetics
Formatted abstract
As in other eukaryotes, a substantial portion of the genome of the human blood flukes belonging to the genus Schistosoma appears to be composed of mobile genetic elements and other repetitive sequences. The constituent elements and their relative organization are not well understood, although retroposons (the SMα elements) and a family of non-long terminal repeat (LTR) retrotransposons (the SR1 elements) have been reported from the genome of Schistosoma mansoni. Here, we report the presence of a second family of non-LTR retrotransposons from S. mansoni which we have termed the SR2 elements. SR2 elements are members of a recently described lineage of non-LTR retrotransposons typified by the RTE-1 non-LTR retrotransposon of Caenorhabditis elegans. We determined the sequence for ~3.9 kb of a consensus full-length SR2 element, which included a long 5′ untranslated region (UTR), potential first and second open reading frames (ORFs) of 78 and 1,018 amino acid residues, respectively, a short 3′ UTR, and an A-rich 3′ terminus. SR2 elements were bound by target site duplications. The putative first and second ORFs did not overlap. The second ORF was homologous to retroviral pol and encoded an apurinic/apyrimidinic endonuclease and a reverse transcriptase. A number of extremely short SR2 elements of less than 0.5 kb, reminiscent of SINEs, were also characterized. These consisted solely of the 5′ and 3′ UTRs of full-length SR2 elements, having both ORFs deleted. Analysis indicated that these SINE-like SR2 elements were produced by replication of a SINE-like SR2 element, rather than by repeated deletions within larger SR2 elements.
© 1999 by the Society for Molecular Biology and Evolution.
Keyword Biochemistry & Molecular Biology
Evolutionary Biology
Genetics & Heredity
Schistosoma Mansoni
Retrotransposon
Ap-endonuclease
Reverse Transcriptase
Non-ltr Retrotransposons
Open Reading Frame
Reverse Transcription
Transposable Elements
Anopheles-gambiae
Sequences
Genes
Site
Rna
Protein
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Tue, 10 Jun 2008, 15:31:22 EST