Papillomavirus capsid protein expression level depends on the match between codon usage and tRNA availability

Zhou, Jian, Liu, Wen Jun, Peng, Shi Wen, Sun, Xiao Yi and Frazer, Ian H. (1999) Papillomavirus capsid protein expression level depends on the match between codon usage and tRNA availability. Journal of Virology, 73 6: 4972-4982.

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Author Zhou, Jian
Liu, Wen Jun
Peng, Shi Wen
Sun, Xiao Yi
Frazer, Ian H.
Title Papillomavirus capsid protein expression level depends on the match between codon usage and tRNA availability
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
Publication date 1999-06
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 73
Issue 6
Start page 4972
End page 4982
Total pages 11
Place of publication Baltimore, USA
Publisher Williams & Wilkins
Collection year 1999
Language eng
Subject C1
320402 Medical Virology
730101 Infectious diseases
Abstract Translation of mRNA encoding the L1 and L2 capsid proteins of papillomavirus (PV) is restricted in vivo to differentiated epithelial cells, although transcription of the L1 and L2 late genes occurs more widely. The codon composition of PV late genes is quite different from that of most mammalian genes. To test the possibility that PV late gene codon composition determines the efficiency of PV late gene expression in some cell types, synthetic bovine papillomavirus type 1 (BPV1) late genes were constructed with codon composition modified to resemble the typical mammalian gene. Expression of these genes from a strong promoter in Cos-1 cells was compared with expression of wild-type BPV1 late genes from the same promoter, Both unmodified and modified PV late genes were transcribed in Cos-l cells, but only the codon-modified genes were translated. In vitro translation of wild-type but not synthetic BPV1 L1 mRNA was markedly enhanced by addition of aminoacyl-tRNAs. Codon composition thus limits BPV1 late gene translation in Cos-l cells, and this limitation can be overcome by modification of the codon composition of the genes or by provision of excess tRNA. Replacement of codons in the green fluorescent protein (gfp) gene with those frequently used in PV late genes did not alter gfp transcription in Cos-l cells but almost abolished translation, supporting the hypothesis that the observed differences in efficiency of translation of modified and unmodified PV capsid genes were related to codon usage rather than mRNA structure. As tRNA populations vary within and between tissues in the same eukaryotic organism, we speculate that matching of tRNA availability to codon usage may be one determinant of the restriction of expression of PV late genes to differentiated epithelium.
Keyword Virology
Virus-like Particles
Rna Instability Elements
Late Messenger-rnas
Bovine Papillomavirus
Cellular Factors
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: UQ Diamantina Institute Publications
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Created: Tue, 10 Jun 2008, 15:24:14 EST