Retinoic acid-dependent upregulation of mouse folate receptor-alpha expression in embryonic stem cells, and conservation of alternative splicing patterns

Bolton, J. A., Wood, S. A., Kennedy, D., Don, R. H. and Mattick, J. S. (1999) Retinoic acid-dependent upregulation of mouse folate receptor-alpha expression in embryonic stem cells, and conservation of alternative splicing patterns. Gene, 230 2: 215-224. doi:10.1016/S0378-1119(99)00081-5


Author Bolton, J. A.
Wood, S. A.
Kennedy, D.
Don, R. H.
Mattick, J. S.
Title Retinoic acid-dependent upregulation of mouse folate receptor-alpha expression in embryonic stem cells, and conservation of alternative splicing patterns
Formatted title
Retinoic acid-dependent upregulation of mouse folate receptor-α expression in embryonic stem cells, and conservation of alternative splicing patterns
Journal name Gene   Check publisher's open access policy
ISSN 0378-1119
1879-0038
Publication date 1999-04-16
Sub-type Article (original research)
DOI 10.1016/S0378-1119(99)00081-5
Volume 230
Issue 2
Start page 215
End page 224
Total pages 10
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Collection year 1999
Language eng
Subject C1
270201 Gene Expression
780105 Biological sciences
Formatted abstract
The action of retinoic acid (RA) in normal development and differentiation is mediated by changes in the expression of RA-responsive target genes. We used differential display reverse transcriptase polymerase chain reaction (RT-PCR) to identify RA-responsive genes expressed in embryonic stem (ES) cells and found that murine folate receptor-α (FR-α) expression is rapidly induced by RA treatment. The observed increase in FR-α expression occurs within 3 h, is independent of protein synthesis and does not occur when ES cells are differentiated by removal of leukaemia inhibitory factor (LIF), evidence that the response to RA is both direct and specific. Two messenger RNA (mRNA) isoforms of FR-α featuring novel sequence in the 5′ untranslated region (UTR) were cloned, and both were found to be upregulated by RA. Other splice variants in both the 5′ UTR and 3′ UTR of FR-α mRNA were also identified. There is a striking similarity between these splicing patterns and those reported for human FR-α, which also generates multiple isoforms by alternative splicing in the 5′ and 3′ UTR. The conservation of these splicing patterns in the non-coding regions of the FR-α gene between mouse and human suggests that these regions, and in particular the 5′ UTR, play an important role in regulating expression of this gene.
© 1999 Elsevier Science B.V. All rights reserved.
Keyword Differentiation
5′ untranslated region
mRNA isoforms
Polymorphism
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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