Serial passage of a Helicoverpa armigera nucleopolyhedrovirus in Helicoverpa zea cell cultures

Chakraborty, S and Reid, S (1999) Serial passage of a Helicoverpa armigera nucleopolyhedrovirus in Helicoverpa zea cell cultures. Journal of Invertebrate Pathology, 73 3: 303-308. doi:10.1006/jipa.1999.4848

Author Chakraborty, S
Reid, S
Title Serial passage of a Helicoverpa armigera nucleopolyhedrovirus in Helicoverpa zea cell cultures
Journal name Journal of Invertebrate Pathology   Check publisher's open access policy
ISSN 0022-2011
Publication date 1999
Sub-type Article (original research)
DOI 10.1006/jipa.1999.4848
Volume 73
Issue 3
Start page 303
End page 308
Total pages 6
Place of publication USA
Publisher Academic
Collection year 1999
Language eng
Subject C1
270800 Biotechnology
780105 Biological sciences
Abstract The serial passaging of baculoviruses in cell lines numerous times can result in a variety of mutations or defective viral populations becoming predominant in the cultures. The generation of these mutants during cell culture passage, also known as the passage effect, can seriously hinder the use of in vitro methods for large-scale production of baculoviruses for use as biopesticides. In an effort to develop a large-scale in vitro method of producing Helicoverpa armigera singly enveloped nucleopolyhedrovirus (HaSNPV), it was essential to determine whether or not the passage effect was evident when this virus is serially passaged in cell cultures. An isolate of HaSNPV was serially passaged in Helicoverpa zea cell, cultures up to 10 times. The production of occlusion bodies decreased with increasing passage number and there was evidence of defective viruses becoming predominant in cultures after 5 passages. The number of virions present within cross sections of passage 3 occlusion bodies was 1.5 times higher than those from passage 10 occlusion bodies when quantified using electron microscopy. A laboratory bioassay showed that potencies of passage 3 isolates against H. armigera larvae were 8 times higher than potencies of passage 10 isolates. This study indicated that changes typical of the passage effect were evident when HaSNPV was serially passaged in H. zea cell cultures up to 10 times. (C) 1999 Academic Press.
Keyword Zoology
Insect Cell Culture
Occlusion Bodies
Few Polyhedra Mutant
Passage Effect
Nuclear Polyhedrosis-virus
In-vitro Production
Plaque Variants
Alfalfa Looper
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemical Engineering Publications
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Citation counts: TR Web of Science Citation Count  Cited 19 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 10 Jun 2008, 14:56:21 EST