Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy

Zheng, YZ, Hyatt, A, Wang, LF, Eaton, BT, Greenfield, PF and Reid, S (1999) Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy. Journal of Virological Methods, 80 1: 1-9.


Author Zheng, YZ
Hyatt, A
Wang, LF
Eaton, BT
Greenfield, PF
Reid, S
Title Quantification of recombinant core-like particles of bluetongue virus using immunosorbent electron microscopy
Journal name Journal of Virological Methods   Check publisher's open access policy
ISSN 0166-0934
Publication date 1999
Sub-type Article (original research)
DOI 10.1016/S0166-0934(98)00170-0
Volume 80
Issue 1
Start page 1
End page 9
Total pages 9
Place of publication The Netherlands
Publisher Elsevier
Collection year 1999
Language eng
Subject C1
270800 Biotechnology
780105 Biological sciences
Abstract Immunosorbent electron microscopy was used to quantify recombinant baculovirus-generated bluetongue virus (BTV) core-like particles (CLP) in either purified preparations or lysates of recombinant baculovirus-infected cells. The capture antibody was an anti-BTV VP7 monoclonal antibody. The CLP concentration in purified preparations was determined to be 6.6 x 10(15) particles/l. CLP concentration in lysates of recombinant baculovirus-infected cells was determined at various times post-infection and shown to reach a value of 3 x 10(15) particles/l of culture medium at 96 h post-infection. The results indicated that immunosorbent electron microscopy, aided by an improved particle counting method, is a simple, rapid and accurate technique for the quantification of virus and virus-like particles produced in large scale in vitro systems. (C) 1999 Elsevier Science B.V. All rights reserved.
Keyword Biochemical Research Methods
Biotechnology & Applied Microbiology
Virology
Immunosorbent Electron Microscopy
Isem
Airfuge
Em-90 Rotor
Bluetongue
Btv Clp
Monoclonal Antibody
Particle Counting
Assay
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Microscopy and Microanalysis Publications
 
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