Survival of spores of the oyster pathogen Marteilia sydneyi (Protozoa, Paramyxea) as assessed using fluorogenic dyes

Wesche, S. J., Adlard, R. D. and Lester, R. J. G. (1999) Survival of spores of the oyster pathogen Marteilia sydneyi (Protozoa, Paramyxea) as assessed using fluorogenic dyes. Diseases of Aquatic Organisms, 36 3: 221-226. doi:10.3354/dao036221

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Author Wesche, S. J.
Adlard, R. D.
Lester, R. J. G.
Title Survival of spores of the oyster pathogen Marteilia sydneyi (Protozoa, Paramyxea) as assessed using fluorogenic dyes
Formatted title
Survival of spores of the oyster pathogen Marteilia sydneyi (protozoa, paramyxea) as assessed using fluorogenic dyes
Journal name Diseases of Aquatic Organisms   Check publisher's open access policy
ISSN 0177-5103
1616-1580
Publication date 1999
Sub-type Article (original research)
DOI 10.3354/dao036221
Open Access Status File (Publisher version)
Volume 36
Issue 3
Start page 221
End page 226
Total pages 6
Place of publication Amelinghausen, Germany
Publisher Inter-Research
Collection year 1999
Language eng
Subject C1
630303 Aquaculture
300702 Pests and Diseases
Formatted abstract
Sporonts of the paramyxean protist Marteilia sydneyi, aetiological agent of 'QX' disease in the Sydney rock oyster Saccostrea commercialis, were isolated from infected oysters and maintained under various physical conditions to assess their survival. Survival was determined using 2 fluorogenic dyes, propidium iodide and 4',6-diamidino-2-phenylindole. The effects of salinity, temperature, ingestion by natural oyster predators, freezing and chlorination were examined. Dye exclusion indicated that spores were short-lived once isolated from the oyster, with the majority dead within 7 to 9 d. Maximum longevity recorded was 35 d at 15°C and 34 ppt. Experiments to investigate the effects of ingestion by fish and birds revealed that spores did not survive for more than 2 h under these conditions, suggesting that it is unlikely that fish or birds play a significant role in the life cycle or dispersal of this parasite. Spores apparently remained viable for over 7 mo when frozen at -20 and -70°C. Chlorine treatments of 200 ppm killed 99.5% of spores within 2 h and all spores within 4 h of exposure.
© Inter-Research 1999
Keyword Fisheries
Veterinary Sciences
Viability
Marteilia Sydneyi
Propidium Iodide
4 ',6-diamidino-2-phenylindole
Qx
Saccostrea Commercialis
Fluorogenic Dyes
Cryptosporidium-parvum Oocysts
Animal Infectivity
Cyst Viability
Excystation
Microscopy
Invitro
Soil
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Tue, 10 Jun 2008, 14:27:18 EST