The extracellular matrix modulates olfactory neurite outgrowth on ensheathing cells

Tisay, Katarina T. and Key, Brian (1999) The extracellular matrix modulates olfactory neurite outgrowth on ensheathing cells. Journal of Neuroscience, 19 22: 9890-9899.

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Author Tisay, Katarina T.
Key, Brian
Title The extracellular matrix modulates olfactory neurite outgrowth on ensheathing cells
Journal name Journal of Neuroscience   Check publisher's open access policy
ISSN 0270-6474
1529-2401
Publication date 1999-11-01
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 19
Issue 22
Start page 9890
End page 9899
Total pages 10
Editor Gordon M. Shepherd
Place of publication Washington, D.C.
Publisher Society of Neuroscience
Collection year 1999
Language eng
Subject C1
270502 Neurobiology
780105 Biological sciences
Abstract Primary olfactory axons grow along a stereotypical pathway from the nasal cavity to the olfactory bulb through an extracellular matrix rich in laminin and heparan sulfate proteoglycans (HSPGs) and bounded by the expression of chondroitin sulfate proteoglycans (CSPGs). This pathway is pioneered by olfactory ensheathing cells, which provide a substrate conducive for axon growth during early development. In the present study, we examined the effect of several extracellular matrix constituents on the spreading and migration, as well as the neurite outgrowth-promoting properties, of olfactory ensheathing cells. Laminin and Matrigel enhanced the spreading and migration of olfactory ensheathing cells and increased their neurite outgrowth-promoting activity. In contrast, HSPG and CSPG had little effect on the spreading and migration of olfactory ensheathing cells and hence did not promote olfactory neurite outgrowth. In vitro olfactory axons grew preferentially on the surface of olfactory ensheathing cells rather than the underlying extracellular matrix. We propose that olfactory ensheathing cells secrete laminin and HSPGs, which together with other cofactors, stimulate these cells to migrate and adopt a neurite outgrowth-promoting phenotype. Expression of CSPGs in the surrounding mesenchyme confines the growth of ensheathing cells, as well as the axons, which grow on the surface of these cells, to a specific pathway. Thus, the ECM indirectly modulates the growth and guidance of olfactory axons during development.
Keyword Neurosciences
Neurite Outgrowth
Laminin
Proteoglycan
Ensheathing Cells
Chondroitin Sulfate
Primary Olfactory Neuron
Nerve-fiber Layer
Heparan-sulfate Proteoglycans
Mammary Epithelial-cells
Schwann-cells
Surface Proteoglycan
Glia Transplants
Axonal Growth
Spinal-cord
Bulb
Regeneration
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Wed, 11 Jun 2008, 00:15:38 EST