Multiple splicing variants of cdc25B regulate G2/M progression

Forrest, A. R. R., McCormack, A. K., DeSouza, C. P. C., Sinnamon, J. M., Tonks, I. D., Hayward, N. K., Ellem, K. A. O. and Gabrielli, B. G. (1999) Multiple splicing variants of cdc25B regulate G2/M progression. Biochemical and Biophysical Research Communications, 260 2: 510-515. doi:10.1006/bbrc.1999.0870


Author Forrest, A. R. R.
McCormack, A. K.
DeSouza, C. P. C.
Sinnamon, J. M.
Tonks, I. D.
Hayward, N. K.
Ellem, K. A. O.
Gabrielli, B. G.
Title Multiple splicing variants of cdc25B regulate G2/M progression
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
1090-2104
Publication date 1999-07-05
Sub-type Article (original research)
DOI 10.1006/bbrc.1999.0870
Volume 260
Issue 2
Start page 510
End page 515
Total pages 6
Place of publication New York, NY, U.S.A.
Publisher Academic Press
Collection year 1999
Language eng
Subject C1
321015 Oncology and Carcinogenesis
730108 Cancer and related disorders
Formatted abstract
Progression through G2 phase into mitosis is regulated by the activation of the mitotic cyclin/cdk complexes, which are in turn activated cdc25B and cdc25C phosphatases. Here we report that alternate splicing produces at least five variants of cdc25B, although only cdc25B2 and cdc25B3 are detectable as proteins. Analysis of these two variants shows that cdc25B2 is expressed at lower levels relative to cdc25B3 in all cell lines tested, and the expression of both increased markedly during G2 and mitosis. Overexpression of the catalytically inactive version of either cdc25B variant produced a G2 arrest implicating both in regulating G2/M progression.
© 1999 Academic Press.
Keyword Biochemistry & Molecular Biology
Biophysics
Cell-cycle
Protein-kinase
Hela-cells
C-myc
Phosphatase
Activation
Phosphorylation
Mitosis
Overexpression
Expression
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 10 Jun 2008, 13:58:02 EST