Novel association of a diverse range of genes with renal cell carcinoma as identified by differential display

Rae, F. K., Stephenson, S. A., Nicol, D. L. and Clements, J. A. (2000) Novel association of a diverse range of genes with renal cell carcinoma as identified by differential display. International Journal of Cancer, 88 5: 726-732. doi:10.1002/1097-0215(20001201)88:5<726::AID-IJC7>3.0.CO;2-H


Author Rae, F. K.
Stephenson, S. A.
Nicol, D. L.
Clements, J. A.
Title Novel association of a diverse range of genes with renal cell carcinoma as identified by differential display
Journal name International Journal of Cancer   Check publisher's open access policy
ISSN 1097-0215
0020-7136
Publication date 2000-12-01
Sub-type Article (original research)
DOI 10.1002/1097-0215(20001201)88:5<726::AID-IJC7>3.0.CO;2-H
Volume 88
Issue 5
Start page 726
End page 732
Total pages 7
Place of publication New York, N.Y. U.S.A.
Publisher Wiley-Liss
Collection year 2000
Language eng
Subject C1
321015 Oncology and Carcinogenesis
730108 Cancer and related disorders
Abstract We have used differential-display FCR (DD-PCR) to compare renal-cell carcinoma (RCC) and normal kidney gene expression with the aim of identifying genes specifically associated with RCC. Using a modified DD-PCR approach, which was non-radioactive, quicker and simpler than the conventional method, 24 cDNA samples were clearly up- or downregulated in RCC tissue from 4 patients. Fourteen of these showed high similarity to a number of known genes. Eight of these cDNA clones were chosen for further analysis. These were a regulator of G-protein signalling (RGS-5), Notch-3, Na,K-ATPase a! subunit, HLA class II antigen, ETS-like protein, transforming growth factor P-stimulated clone (TSC-22), bladder cancer-related protein (BC10) and adipophilin. Semi-quantitative RT-PCR using specific primers to each of these genes confirmed differential expression in 67% to 83% of a further 12 RCC and normal kidney paired samples from 7 of the 8 cDNA clones. Northern analysis further confirmed the up-regulation in expression of RGS-5 and Notch-3 in RCC. Further characterisation of these differentially expressed genes should lead to a better understanding of the changes that occur at the molecular level during RCC development and progression. (C) 2000 Wiley-Liss, Inc.
Keyword Oncology
Heterotrimeric G-proteins
Tumor-suppressor Gene
Down-regulation
Rgs Proteins
Tissue Expression
Human Homolog
Cancer
Protooncogene
Polymorphism
Inhibition
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Tue, 10 Jun 2008, 13:20:59 EST