Preliminary functional analysis of human epidermal T cells

Sugerman, Philip B. and Bigby, Michael (2000) Preliminary functional analysis of human epidermal T cells. Archives of Dermatological Research, 292 1: 9-15. doi:10.1007/PL00007461

Author Sugerman, Philip B.
Bigby, Michael
Title Preliminary functional analysis of human epidermal T cells
Journal name Archives of Dermatological Research   Check publisher's open access policy
ISSN 0340-3696
Publication date 2000-01
Sub-type Article (original research)
DOI 10.1007/PL00007461
Volume 292
Issue 1
Start page 9
End page 15
Total pages 7
Place of publication Berlin, Germany
Publisher Springer-Verlag
Collection year 2000
Language eng
Subject C1
320899 Dentistry not elsewhere classified
730112 Oro-dental and disorders
Formatted abstract
The function of human epidermal T cells (ETC) is unknown. In the present study, dermal T cells (DTC), ETC and keratinocytes were cultured from normal human skin, DTC and ETC lines were expanded in medium containing interleukin 2. The autologous keratinocytes were transfected with a human papillomavirus 16 E6 and E7 plasmid to produce an immortal keratinocyte line 'HEK001'. Lymphocyte migration and adhesion to HEK001 was assessed in calcein fluorimetric assays, ETC migrated towards HEK001 three to four times more than DTC. ETC adhered to HEK001 two to four times more than DTC. The proportion of ETC expressing the cutaneous lymphocyte-associated antigen was greater than that of DTC (26% and 1%, respectively). The keratinocyte line HEK001 expressed ICAM-1 following stimulation with TNF-α or IFN-γ and following coculture with autologous cutaneous T cells, A blocking anti-ICAM-1 antibody reduced DTC and ETC adhesion to HEK001 by 30% and 50%, respectively. Therefore, cutaneous T cells may upregulate keratinocyte ICAM-1 expression which mediates adhesion to autologous keratinocytes. These results are consistent with the hypothesis that the ETC and DTC populations are distinct. Both directed migration (epidermotropism) and selective retention may be involved in the development and maintenance of the ETC population in normal human skin.
Keyword Dermatology
Epidermal T Cells
Normal Human-skin
Human Keratinocytes
Thy-1 Antigen
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Dentistry Publications
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Created: Tue, 10 Jun 2008, 13:09:42 EST