Sequence analysis and functional characterization of the violacein biosynthetic pathway from Chromobacterium violaceum

August, P. R., Grossman, T. H., Minor, C., Draper, M. P., MacNeil, I. A., Pemberton, J. M., Call, K. M., Holt, D. and Osburne, M. S. (2000) Sequence analysis and functional characterization of the violacein biosynthetic pathway from Chromobacterium violaceum. Journal of Molecular Microbiology and Biotechnology, 2 4: 513-519.

Author August, P. R.
Grossman, T. H.
Minor, C.
Draper, M. P.
MacNeil, I. A.
Pemberton, J. M.
Call, K. M.
Holt, D.
Osburne, M. S.
Title Sequence analysis and functional characterization of the violacein biosynthetic pathway from Chromobacterium violaceum
Journal name Journal of Molecular Microbiology and Biotechnology   Check publisher's open access policy
ISSN 1464-1801
Publication date 2000-01-01
Sub-type Article (original research)
Volume 2
Issue 4
Start page 513
End page 519
Total pages 7
Place of publication UK
Publisher Horizon Scientific Press
Collection year 2000
Language eng
Subject C3
270306 Microbial Genetics
780105 Biological sciences
Abstract Violacein is a purple-colored, broad-spectrum antibacterial pigment that has a dimeric structure composed of 5-hydroxyindole, oxindole and 2-pyyrolidone subunits formed by the condensation of two modified tryptophan molecules. The violacein biosynthetic gene cluster from Chromobacterium violaceum was characterized by DNA sequencing, transposon mutagenesis, and chemical analysis of the pathway intermediates produced heterologously in Escherichia. coli, The violacein biosynthetic gene cluster spans eight kilobases and is comprised of the four genes, vioABCD, that are necessary for violacein production. Sequence analysis suggests that the products of vioA, vioC and vioD are nucleotide-dependent monooxygenases. Disruption of vioA or vice completely abrogates the biosynthesis of violacein intermediates, while disruption of the vioC or vioD genes results in the production of violacein precursors.
Keyword Biotechnology & Applied Microbiology
Microbiology
Acyl Carrier Proteins
Molecular-cloning
Aplysia-kurodai
Gene-cluster
Sea Hare
Streptomyces
Expression
Synthase
Tool
Q-Index Code C3

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 80 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 10 Jun 2008, 22:47:27 EST