Aerobic nitrate respiration in a nitrite-oxidising bioreactor

McDevitt, C., Burrell, P., Blackall, L. L. and McEwan, A. G. (2000) Aerobic nitrate respiration in a nitrite-oxidising bioreactor. FEMS Microbiology Letters, 184 1: 113-118. doi:10.1111/j.1574-6968.2000.tb09000.x


Author McDevitt, C.
Burrell, P.
Blackall, L. L.
McEwan, A. G.
Title Aerobic nitrate respiration in a nitrite-oxidising bioreactor
Journal name FEMS Microbiology Letters   Check publisher's open access policy
ISSN 0378-1097
Publication date 2000
Sub-type Article (original research)
DOI 10.1111/j.1574-6968.2000.tb09000.x
Volume 184
Issue 1
Start page 113
End page 118
Total pages 6
Place of publication North Holland
Publisher Elsevier
Collection year 2000
Language eng
Subject C1
270307 Microbial Ecology
770502 Land and water management
780105 Biological sciences
Abstract The ability of heterotrophic bacteria in a nitrite-oxidising bioreactor to respire with nitrate as an electron acceptor was examined. Approximately 70% of 1000 heterotrophic isolates were able to express a nitrate reductase. A detailed survey of 15 isolates showed that five expressed the azide-insensitive nitrate reductase encoded by the napA gene. A two-round PCR amplification of the napA gene using degenerate PCR primers and DNA sequence analysis of these products confirmed the presence of this gene in the positive isolates. Partial 16S rDNA products and napA products were amplified from the biomass in the bioreactor and denaturing gradient gel electrophoresis of these products identified 21 distinct ribotypes and 12 distinct napA sequences. The results show that the ability to respire with nitrate as an electron acceptor under aerobic conditions is widespread among the heterotrophic population of this bioreactor. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
Keyword Microbiology
Nitrate Respiration
Nitrite Oxidation
Heterotrophic Bacteria
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemistry and Molecular Biosciences
Advanced Water Management Centre Publications
 
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