Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and is required for late endosome-lysosome fusion

Mullock, Barbara M. M, Smith, Chez W., Ihrke, Gudrun, Bright, Nicholas A., Lindsay, Margaret, Parkinson, Emma J., Brooks, Doug A., Parton, Robert G., James, David E., Luzio, J. Paul and Piper, Robert C. (2000) Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and is required for late endosome-lysosome fusion. Molecular Biology of the Cell, 11 9: 3137-3153.

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Author Mullock, Barbara M. M
Smith, Chez W.
Ihrke, Gudrun
Bright, Nicholas A.
Lindsay, Margaret
Parkinson, Emma J.
Brooks, Doug A.
Parton, Robert G.
James, David E.
Luzio, J. Paul
Piper, Robert C.
Title Syntaxin 7 is localized to late endosome compartments, associates with Vamp 8, and is required for late endosome-lysosome fusion
Journal name Molecular Biology of the Cell   Check publisher's open access policy
ISSN 1059-1524
1939-4586
Publication date 2000-09
Sub-type Article (original research)
Open Access Status File (Publisher version)
Volume 11
Issue 9
Start page 3137
End page 3153
Total pages 17
Place of publication Bethesda, MD, United States
Publisher American Society for Cell Biology
Collection year 2000
Language eng
Formatted abstract
Protein traffic from the cell surface or the trans-Golgi network reaches the lysosome via a series of endosomal compartments. One of the last steps in the endocytic pathway is the fusion of late endosomes with lysosomes. This process has been reconstituted in vitro and has been shown to require NSF, α and γ SNAP, and a Rab GTPase based on inhibition by Rab GDI. In Saccharomyces cerevisiae, fusion events to the lysosome-like vacuole are mediated by the syntaxin protein Vam3p, which is localized to the vacuolar membrane. In an effort to identify the molecular machinery that controls fusion events to the lysosome, we searched for mammalian homologues of Vam3p. One such candidate is syntaxin 7. Here we show that syntaxin 7 is concentrated in late endosomes and lysosomes. Coimmunoprecipitation experiments show that syntaxin 7 is associated with the endosomal V-SNARE Vamp 8, which partially colocalizes with syntaxin 7. Importantly, we show that syntaxin 7 is specifically required for the fusion of late endosomes with lysosomes in vitro, resulting in a hybrid organelle. Together, these data identify a SNARE complex that functions in the late endocytic system of animal cells.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Created: Tue, 10 Jun 2008, 12:25:38 EST