UNCL, the mammalian homologue of UNC-50, is an inner nuclear membrane RNA-binding protein

Fitzgerald, J., Kennedy, D., Viseshakul, N., Cohen, B. N., Mattick, J., Bateman, J. F. and Forsayeth, J. R. (2000) UNCL, the mammalian homologue of UNC-50, is an inner nuclear membrane RNA-binding protein. Brain Research, 877 1: 110-123. doi:10.1016/S0006-8993(00)02692-5


Author Fitzgerald, J.
Kennedy, D.
Viseshakul, N.
Cohen, B. N.
Mattick, J.
Bateman, J. F.
Forsayeth, J. R.
Title UNCL, the mammalian homologue of UNC-50, is an inner nuclear membrane RNA-binding protein
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
1872-6240
Publication date 2000-09
Sub-type Article (original research)
DOI 10.1016/S0006-8993(00)02692-5
Volume 877
Issue 1
Start page 110
End page 123
Total pages 14
Place of publication Amsterdam
Publisher Elsevier Science
Collection year 2000
Language eng
Subject C1
270106 Cell Development (incl. Cell Division and Apoptosis)
780105 Biological sciences
1109 Neurosciences
1702 Cognitive Sciences
Abstract We isolated a mammalian homologue of the C. elegans gene unc-50 that Lye have named UNCL. The 777 kb rat UNCL cDNA encodes a 259 amino acid protein that is expressed in a wide variety of tissues with highest mRNA levels in brain, kidney and testis. Hydropathy plot analysis and in vitro translation experiments with microsomal membranes indicate that UNCL is a transmembrane protein. Hemagglutinin tagged UNCL was stably transfected into SaOS-2 osteosarcoma cells and exhibited a nuclear rim staining pattern which was retained following extraction with 1% Triton X-100, suggesting that UNCL localizes to the inner nuclear membrane. UNCL-HA was extractable in 350 mM NaCl, suggesting that UNCL is not associated with the nuclear matrix. Homopolymer RNA-binding assays performed on in vitro translated UNCL protein and 'structural modeling by homology' suggest that UNCL binds RNA via an amino-terminal RNA Recognition-like Motif. Since unc-50 is required for expression of assembled muscle-type nicotinic receptors in the nematode we investigated whether UNCL had a similar function for mammalian nicotinic receptors. When UNCL was co-expressed with neural nicotinic receptors in Xenopus oocytes or COS cells it increased expression of functional cell surface receptors up to 1.6-fold. We conclude that UNCL is a novel inner nuclear membrane protein that associates with RNA and is involved in the cell-surface expression of neuronal nicotinic receptors. UNCL plays a broader role because UNCL homologues are present in two yeast and a plant species, none of which express nicotinic receptors and it is also found in tissues that lack nicotinic receptors. (C) 2000 Elsevier Science B.V. All rights reserved.
Keyword Neurosciences
Neuronal Nachr
Rna-binding
Inner Nuclear Membrane
Unc-50
Dreifuss Muscular-dystrophy
Lamin-b-receptor
Nicotinic Acetylcholine-receptors
Nematode Caenorhabditis-elegans
Messenger-rna
Endoplasmic-reticulum
Recognition Motif
Crystal-structure
Cos Cells
Envelope
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Brain Institute Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 26 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 28 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 10 Jun 2008, 11:57:38 EST