Isolation of a funnel-web spider polypeptide with homology to mamba intestinal toxin 1 and the embryonic head inducer Dickkopf-1

Szeto, T. H., Wang, X. H., Smith, R., Connor, M., Christie, M. J., Nicholson, G. M. and King, G. F. (2000) Isolation of a funnel-web spider polypeptide with homology to mamba intestinal toxin 1 and the embryonic head inducer Dickkopf-1. Toxicon, 38 3: 429-442. doi:10.1016/S0041-0101(99)00174-9


Author Szeto, T. H.
Wang, X. H.
Smith, R.
Connor, M.
Christie, M. J.
Nicholson, G. M.
King, G. F.
Title Isolation of a funnel-web spider polypeptide with homology to mamba intestinal toxin 1 and the embryonic head inducer Dickkopf-1
Journal name Toxicon   Check publisher's open access policy
ISSN 0041-0101
1879-3150
Publication date 2000
Sub-type Article (original research)
DOI 10.1016/S0041-0101(99)00174-9
Volume 38
Issue 3
Start page 429
End page 442
Total pages 14
Editor Alan L. Harvey
Place of publication Oxford, United Kingdom
Publisher Elsevier Science
Collection year 2000
Language eng
Subject C1
270100 Biochemistry and Cell Biology
780105 Biological sciences
1115 Pharmacology and Pharmaceutical Sciences
111506 Toxicology (incl.Clinical Toxicology)
Abstract We have isolated and determined the amino acid sequence of a novel peptide component from the venom of the Australian funnel-web spider Hadronyche versuta. This 68-residue toxin, ACTX-Hvf17, does not function like classical neurotoxins in modulating ion channel function as evidenced by its lack of insecticidal activity and its inability to affect vertebrate smooth or skeletal muscle contractility. The peptide shows significant sequence homology with mamba intestinal toxin 1 (MIT1) and to a lesser extent with a variety of colipases. The strong structural homology between MIT1 and porcine colipase leads us to propose that ACTX-Hvf17 also adopts the MIT1/colipase three-dimensional fold. However, we show that ACTX-Hvf17 has no colipase activity and does not stimulate muscle contractility like MIT1. We also show that MIT1 and ACTX-Hvf17 display significant sequence homology with the C-terminal cysteine-rich domain of the Dickkopf-1 family of proteins that induce head formation in developing embryos, which leads us to propose that this domain of Dickkopf-1 also adopts the MIT1/colipase fold. (C) 1999 Elsevier Science Ltd. All rights reserved.
Keyword Australian funnel-web spider
Adronyche versuta
Novel peptide component
Amino acid sequence isolation
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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