A pore-lining glutamic acid in the rat olfactory cyclic nucleotide-gated channel controls external spermine block

Nevin, Simon T., Haddrill, Justine L. and Lynch, Joseph W. (2000) A pore-lining glutamic acid in the rat olfactory cyclic nucleotide-gated channel controls external spermine block. Neuroscience Letters, 296 2-3: 163-167.


Author Nevin, Simon T.
Haddrill, Justine L.
Lynch, Joseph W.
Title A pore-lining glutamic acid in the rat olfactory cyclic nucleotide-gated channel controls external spermine block
Journal name Neuroscience Letters   Check publisher's open access policy
ISSN 0304-3940
Publication date 2000-12-22
Sub-type Article (original research)
DOI 10.1016/S0304-3940(00)01650-5
Volume 296
Issue 2-3
Start page 163
End page 167
Total pages 5
Editor M. Zimmermann
Place of publication Clare, Ireland
Publisher Elsevier Science Ireland
Collection year 2000
Language eng
Subject C1
270104 Membrane Biology
780105 Biological sciences
Abstract Spermine is a potent, voltage-dependent blocker of the olfactory cyclic nucleotide-gated channel from both the intracellular and extracellular sides. However, its sites of action are unknown. This study investigated the external spermine binding site in the rat CNCgreek small letter alpha3 subunit. Neutralization of a glutamic acid residue (E342Q) in the P-loop region eliminated voltage-dependence of block by externally applied spermine. The charge-conservative E342D mutation had little effect on spermine block. Thus, E342 forms the binding site for externally applied spermine. However, spermine remained a potent voltage-independent blocker of the E342Q mutant channel, suggesting that the mutation either created a novel binding site outside the membrane electrical field or that it dramatically changed the properties of the existing pore site.
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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