Stability of sirolimus (Rapamycin) in whole blood

Salm, P., Tresillian, M. J., Taylor, P. J. and Pillans, P. I. (2000) Stability of sirolimus (Rapamycin) in whole blood. Therapeutic Drug Monitoring, 22 4: 423-426. doi:10.1097/00007691-200008000-00010


Author Salm, P.
Tresillian, M. J.
Taylor, P. J.
Pillans, P. I.
Title Stability of sirolimus (Rapamycin) in whole blood
Journal name Therapeutic Drug Monitoring   Check publisher's open access policy
ISSN 0163-4356
Publication date 2000
Sub-type Article (original research)
DOI 10.1097/00007691-200008000-00010
Volume 22
Issue 4
Start page 423
End page 426
Total pages 4
Place of publication Philadelphia, USA
Publisher Lippincott Williams & Wilkins
Collection year 2000
Language eng
Subject C1
320501 Pharmaceutical Sciences and Pharmacy
730305 Diagnostic methods
1115 Pharmacology and Pharmaceutical Sciences
Abstract The effects of storage time (0 - 8 days), temperature (4 degrees C and 30 degrees C in dark and light), and freeze-thaw cycles on the stability of sirolimus in blood were examined. Sirolimus quantification was undertaken using HPLC-electrospray-tandem mass spectrometry. Whole blood samples supplemented with sirolimus (5.0, 15.0, and 30.0 mu g/L) and pooled renal and heart transplant samples were found to be stable during the 8 days under all conditions (<10% decrease in concentration). No significant difference was observed in sirolimus concentration between freshly collected patient samples and sirolimus-supplemented samples (5.0, 15.0, and 30.0 mu g/L) after three freeze-thaw cycles (p > 0.198). In conclusion, blood samples can be transported with or without cooling for up to 8 days without sirolimus results being compromised. The reanalysis of sirolimus samples, which may entail freeze-thaw cycles, can be undertaken if the number of cycles is three or less.
Keyword Medical Laboratory Technology
Pharmacology & Pharmacy
Toxicology
Sirolimus
Freeze-thaw Sability
Storage Stability
Therapeutic Drug Monitoring
Mass-spectrometry
Electrospray
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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Created: Tue, 10 Jun 2008, 11:09:57 EST