Detection of male DNA in the liver of female patients with primary biliary cirrhosis

Fanning, Peter A., Jonsson, Julie R., Clouston, Andrew D., Edwards-Smith, Cathy, Balderson, Glenda A., Macdonald, Graeme A., Crawford, Darrell H. G., Kerlin, Paul, Powell, Lawrie W. and Powell, Elizabeth E. (2000) Detection of male DNA in the liver of female patients with primary biliary cirrhosis. Journal of Hepatology, 33 5: 690-695. doi:10.1016/S0168-8278(00)80297-4


Author Fanning, Peter A.
Jonsson, Julie R.
Clouston, Andrew D.
Edwards-Smith, Cathy
Balderson, Glenda A.
Macdonald, Graeme A.
Crawford, Darrell H. G.
Kerlin, Paul
Powell, Lawrie W.
Powell, Elizabeth E.
Title Detection of male DNA in the liver of female patients with primary biliary cirrhosis
Journal name Journal of Hepatology   Check publisher's open access policy
ISSN 0168-8278
Publication date 2000
Sub-type Article (original research)
DOI 10.1016/S0168-8278(00)80297-4
Volume 33
Issue 5
Start page 690
End page 695
Total pages 6
Place of publication Netherlands
Publisher Elsevier Science
Collection year 2000
Language eng
Subject C1
321006 Gastroenterology and Hepatology
730113 Digestive system and disorders
Abstract Background/Aims: Primary biliary cirrhosis is a chronic cholestatic liver disease characterized by progressive inflammatory destruction of bile ducts, with eventual hepatic fibrosis and cirrhosis, Since primary biliary cirrhosis affects predominantly middle-aged women and has pathological similarities to hepatic graft-versus-host-disease, we investigated whether fetal cell microchimerism might be involved in the development of this disease. Methods: The presence of Y-chromosome-specific sequences was analyzed by polymerase chain reaction using peripheral blood mononuclear cells from women with primary biliary cirrhosis (n=18) and healthy (control) women (n=18), and by in situ hybridization of liver biopsy sections from women with primary biliary cirrhosis (n=19) and women with chronic hepatitis C or alcoholic liver disease (n=20). Results: Male cells were detected in liver biopsy specimens of 8 of 19 patients (42%) with primary biliary cirrhosis, Y-chromosome-containing cells were not seen in any of the liver biopsy specimens from women with chronic hepatitis C or alcoholic liver disease, Male cells were detected in peripheral blood mononuclear cells from one healthy control at a level of 1 male cell per 10(6) female cells, but were not detected in peripheral blood mononuclear cells of women with primary biliary cirrhosis. Conclusions: The presence of male cells in the liver of women with primary biliary cirrhosis raises the possibility that fetal cell microchimerism may be involved in the pathogenesis of this chronic liver disease.
Keyword Gastroenterology & Hepatology
Autoimmune Disease
Fetal Cell
Microchimerism
Pregnancy
Versus-host Disease
Dendritic Cells
Peripheral-blood
Bile-duct
Women
Transplantation
Scleroderma
Antibodies
Autoimmune
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 60 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 69 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Tue, 10 Jun 2008, 11:05:49 EST