The nucleotide sequence of Koala (Phascolarctos cinereus) retrovirus: a novel type C endogenous virus related to Gibbon Ape leukemia virus

Hanger, Jon J., Bromham, Lindell D., McKee, Jeff J., OBrien, Tracy M. and Robinson, Wayne F. (2000) The nucleotide sequence of Koala (Phascolarctos cinereus) retrovirus: a novel type C endogenous virus related to Gibbon Ape leukemia virus. Journal of Virology, 74 9: 4264-4272. doi:10.1128/JVI.74.9.4264-4272.2000

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Author Hanger, Jon J.
Bromham, Lindell D.
McKee, Jeff J.
OBrien, Tracy M.
Robinson, Wayne F.
Title The nucleotide sequence of Koala (Phascolarctos cinereus) retrovirus: a novel type C endogenous virus related to Gibbon Ape leukemia virus
Journal name Journal of Virology   Check publisher's open access policy
ISSN 0022-538X
1098-5514
Publication date 2000-05-01
Year available 2000
Sub-type Article (original research)
DOI 10.1128/JVI.74.9.4264-4272.2000
Open Access Status File (Publisher version)
Volume 74
Issue 9
Start page 4264
End page 4272
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2000
Language eng
Subject C1
300510 Virology
770703 Living resources (flora and fauna)
Abstract A novel retrovirus, morphologically consistent,vith mammalian C-type retroviruses, was detected by electron microscopy in mitogen-stimulated peripheral blood mononuclear cell cultures from 163 koalas and in lymphoma tissue from 3 koalas. PCR amplified provirus from the blood and tissues of 17 wild and captive koalas, and reverse transcriptase-PCR demonstrated viral mRNA, viral genomic RNA, and reverse transcriptase activity in koala serum and cell culture supernatants, Comparison of viral sequences derived from genomic DNA and mRNA showed identity indicative of a single retroviral species-here designated koala retrovirus (KoRV), Southern blot analysis of koala tissue genomic DNA using labelled KoRV probes demonstrated banding consistent with an endogenous retrovirus. Complete and apparently truncated proviruses were detected in DNA of both clinically normal koalas and those with hematopoietic disease. KoRV-related viruses were not detected in other marsupials, and phylogenetic analysis showed that KoRV paradoxically clusters with gibbon ape leukemia virus (GALV). The strong similarity between GALV and KoRV suggests that these viruses are closely related and that recent cross-host transmission has occurred. The complete proviral DNA sequence of KoRV is reported.
Keyword Virology
Infected Cell-line
Lymphoid Neoplasia
Spectral-analysis
Phylogenetic Data
Messenger-rna
Expression
Dna
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Tue, 10 Jun 2008, 10:59:52 EST