Albicidin antibiotic and phytotoxin biosynthesis in Xanthomonas albilineans requires a phosphopantetheinyl transferase gene

Huang, GZ, Zhang, LH and Birch, RG (2000) Albicidin antibiotic and phytotoxin biosynthesis in Xanthomonas albilineans requires a phosphopantetheinyl transferase gene. Gene, 258 1-2: 193-199.


Author Huang, GZ
Zhang, LH
Birch, RG
Title Albicidin antibiotic and phytotoxin biosynthesis in Xanthomonas albilineans requires a phosphopantetheinyl transferase gene
Journal name Gene   Check publisher's open access policy
Publication date 2000
Sub-type Article
DOI 10.1016/S0378-1119(00)00451-0
Volume number 258
Issue number 1-2
ISSN 0378-1119
Start page 193
End page 199
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier Science BV
Collection year 2000
Language eng
Subject C1
270800 Biotechnology
780105 Biological sciences
Abstract Xanthomonas albilineans produces a family of highly potent antibiotics and phytotoxins called albicidins, which are key pathogenesis factors in the systemic development of leaf scald disease of sugarcane. A gene (xabA) required for albicidin biosynthesis encodes a peptide of 278 amino acids, including the signature sequence motifs for phosphopantetheinyl transferases (PPTases) that activate polyketide and non-ribosomal peptide synthetases. The Escherichia coli entD gene, which encodes a PPTase involved in biosynthesis of enterobactin (a siderophore), restored biosynthesis of albicidin (a DNA replication inhibitor) in X. albilineans Tox(-) LS156 (xabA::Tn5). We conclude that XabA is a PPTase required for post-translational activation of synthetases in the albicidin biosynthetic pathway. This is the first antibiotic or toxin biosynthesis gene characterized from the genus Xanthomonas, and the first demonstration that antibiotic synthetases in the Pseudomonadaceae, like those in the Enterobacteriaceae and in Gram-positive bacteria, can require activation by a PPTase. Coupled with the recent demonstration of a separate albicidin biosynthetic gene cluster, the results indicate the possibility for overproduction of albicidins, which allows better understanding and application of these potent inhibitors of prokaryote DNA replication. (C) 2000 Elsevier Science B.V. All rights reserved.
Keyword Genetics & Heredity
Albicidin Antibiotic And Phytotoxin
Biosynthesis
Phosphopantetheinyl Transferase
Escherichia-coli
Bacillus-subtilis
Polyketide
Sugarcane
Peptide
Detoxification
Synthetases
Resistance
Chlorosis
Synthases
Q-Index Code C1

Document type: Journal Article
Sub-type: Article
Collection: School of Biological Sciences Publications
 
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Created: Tue, 10 Jun 2008, 10:45:57 EST