Latent stem and progenitor cells in the hippocampus are activated by neural excitation

Walker, Tara L., White, Amanda, Black, Debra M., Wallace, Robyn H., Sah, Pankaj and Bartlett, Perry F. (2008) Latent stem and progenitor cells in the hippocampus are activated by neural excitation. Journal of Neuroscience, 28 20: 5240-5247. doi:10.1523/JNEUROSCI.0344-08.2008

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Author Walker, Tara L.
White, Amanda
Black, Debra M.
Wallace, Robyn H.
Sah, Pankaj
Bartlett, Perry F.
Title Latent stem and progenitor cells in the hippocampus are activated by neural excitation
Journal name Journal of Neuroscience   Check publisher's open access policy
ISSN 0270-6474
1529-2401
Publication date 2008-05-14
Sub-type Article (original research)
DOI 10.1523/JNEUROSCI.0344-08.2008
Open Access Status File (Publisher version)
Volume 28
Issue 20
Start page 5240
End page 5247
Total pages 8
Editor John H.R. Maunsell
Place of publication Washington , D.C.
Publisher Neuroscience Society
Collection year 2009
Language eng
Subject C1
1109 Neurosciences
920111 Nervous System and Disorders
Abstract The regulated production of neurons in the hippocampus throughout life underpins important brain functions such as learning and memory. Surprisingly, however, studies have so far failed to identify a resident hippocampal stem cell capable of providing the renewable source of these neurons. Here, we report that depolarizing levels of KCl produce a threefold increase in the number of neurospheres generated from the adult mouse hippocampus. Most interestingly, however, depolarizing levels of KCl led to the emergence of a small subpopulation of precursors (approximately eight per hippocampus) with the capacity to generate very large neurospheres (>250 µm in diameter). Many of these contained cells that displayed the cardinal properties of stem cells: multipotentiality and self-renewal. In contrast, the same conditions led to the opposite effect in the other main neurogenic region of the brain, the subventricular zone, in which neurosphere numbers decreased by ~40% in response to depolarizing levels of KCl. Most importantly, we also show that the latent hippocampal progenitor population can be activated in vivo in response to prolonged neural activity found in status epilepticus. This work provides the first direct evidence of a latent precursor and stem cell population in the adult hippocampus, which is able to be activated by neural activity. Because the latent population is also demonstrated to reside in the aged animal, defining the precise mechanisms that underlie its activation may provide a means to combat the cognitive deficits associated with a decline in neurogenesis.
Keyword hippocampus
potassium
depolarization
activation
precursor
stem cell
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes This is a duplicate of UQ:167341 which has been deleted

 
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Created: Fri, 16 May 2008, 15:37:37 EST by Belinda Weaver on behalf of Queensland Brain Institute