Identification and isolation of synovial dendritic cells

Pettit, Allison R., Cavanagh, Lois, Boyce, Amanda, Padmanabha, Jagadish, Peng, Judy and Thomas, Ranjeny. (2007). Identification and isolation of synovial dendritic cells. In Andrew P. Cope (Ed.), Arthritis Research Methods and Protocols (pp. 165-181) Totowa, N.J., U.S.A.: Humana Press Inc.

Author Pettit, Allison R.
Cavanagh, Lois
Boyce, Amanda
Padmanabha, Jagadish
Peng, Judy
Thomas, Ranjeny.
Title of chapter Identification and isolation of synovial dendritic cells
Title of book Arthritis Research Methods and Protocols
Place of Publication Totowa, N.J., U.S.A.
Publisher Humana Press Inc
Publication Year 2007
Sub-type Research book chapter (original research)
Series Methods in molecular medicine ; 136
ISBN 9781588299185
Editor Andrew P. Cope
Volume number 2
Chapter number 12
Start page 165
End page 181
Total pages 17
Total chapters 27
Collection year 2008
Language eng
Subjects B1
320207 Autoimmunity
730102 Immune system and allergy
1103 Clinical Sciences
0304 Medicinal and Biomolecular Chemistry
Abstract/Summary In rheumatoid arthritis patients, three compartments need to be considered: peripheral blood, synovial fluid, and synovial tissue. Dendritic cells characterized from each compartment have different properties. The methods given are based on cell sorting for isolation of cells, and flow cytometry and immunohistochemical staining for analysis of cells in these compartments.
Keyword Dendritic cells
synovial tissue
synovial fluid
peripheral blood
rheumatoid arthritis
flow cytometry
immunohistochemistry
Q-Index Code B1
Q-Index Status Confirmed Code

 
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Created: Thu, 15 May 2008, 16:07:48 EST by Kylie Hengst on behalf of UQ Diamantina Institute