Secretory signal sequence non-optimal codons are required for expression and export of beta-lactamase

Zalucki, Y., Gittins, K. and Jennings, M. (2008) Secretory signal sequence non-optimal codons are required for expression and export of beta-lactamase. Biochemical and Biophysical Research Communications, 366 1: 135-141. doi:10.1016/j.bbrc.2007.11.093


Author Zalucki, Y.
Gittins, K.
Jennings, M.
Title Secretory signal sequence non-optimal codons are required for expression and export of beta-lactamase
Formatted title
Secretory signal sequence non-optimal codons are required for expression and export of β-lactamase
Journal name Biochemical and Biophysical Research Communications   Check publisher's open access policy
ISSN 0006-291X
1090-2104
Publication date 2008-01-01
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.bbrc.2007.11.093
Volume 366
Issue 1
Start page 135
End page 141
Total pages 7
Place of publication United States of America
Publisher Academic Press
Collection year 2008
Language eng
Subject 270201 Gene Expression
270301 Bacteriology
C1
780105 Biological sciences
Abstract In this study we altered the codon usage in the signal sequence of the bla gene, encoding beta-lactamase in Escherichia coli. Changing all of the thirteen non-optimal codons to optimal lowered expression 4-fold as measured by minimum inhibitory concentration (MIC) to the beta-lactam antibiotic ampicillin. The difference in ampicillin resistance was reduced at 28 degrees C compared to expression at 37 degrees C, suggesting that the optimised bla allele is misfolded and degraded by heat-shock regulated proteases. A screen was carried out, designed specifically to identify revertants with changes in codon usage resulting in higher MIC to ampicillin. The nine revertants revealed by this method all had optimal to non-optimal codon changes in the signal sequence. These results, and those of our previous study with maltose binding protein model system, confirm that non-optimal codons are important for expression and export of secretory proteins via both the SecB-dependent and -independent pathways.
Keyword Protein export
Codon usage
Signal sequence
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Available online 29 November 2007

 
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Created: Tue, 13 May 2008, 14:12:21 EST by Darryl Greensill on behalf of School of Chemistry & Molecular Biosciences