Purification of immunoglobulins from chicken sera by thiophilic gel chromatography

Constantinoiu, C. C., Molloy, J. B., Jorgensen, W. K. and Coleman, G. T. (2007) Purification of immunoglobulins from chicken sera by thiophilic gel chromatography. Poultry Science, 86 9: 1910-1914.

Author Constantinoiu, C. C.
Molloy, J. B.
Jorgensen, W. K.
Coleman, G. T.
Title Purification of immunoglobulins from chicken sera by thiophilic gel chromatography
Journal name Poultry Science   Check publisher's open access policy
ISSN 1525-3171
Publication date 2007-09
Sub-type Article (original research)
Volume 86
Issue 9
Start page 1910
End page 1914
Total pages 5
Editor S. Pollock
C. Scanes
Place of publication USA
Publisher Poultry Science Association
Collection year 2008
Language eng
Subject C1
300504 Immunology
630106 Poultry
Abstract Immunoglobulin Y is different from most of the other immunoglobulins because it does not bind protein A or protein G. Thiophilic gel chromatography has been successfully used to purify IgY from chicken egg yolk, but the technology has not previously been used to purify IgY from serum. In this research note, we describe the optimization of T-gel chromatography for purification of IgY from serum. Data are provided on the recovery and purity of IgY obtained using potassium sulfate buffers of different concentrations. Decreasing the strength of potassium sulfate buffer from 0.5 to 0.3 M did not alter the amount of IgY recovered but increased the purity. Using 0.3 M potassium sulphate, we recovered approximately 63.7% of the serum Ig as almost pure IgY.
Keyword immunoglobulin Y
thiophilic chromatography
Q-Index Code C1
Q-Index Status Confirmed Code

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
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Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 13 May 2008, 10:42:27 EST by Mrs Christine Wheatland on behalf of School of Veterinary Science