A bioinformatic approach to the identification of a conserved domain in a sugarcane legumain that directs GFP to the lytic vacuole

Jackson, M. A., Rae, A. L., Casu, R. E., Grof, C. P. L., Bonnett, G. D. and Maclean, D. J. (2007) A bioinformatic approach to the identification of a conserved domain in a sugarcane legumain that directs GFP to the lytic vacuole. Functional Plant Biology, 34 7: 633-644.


Author Jackson, M. A.
Rae, A. L.
Casu, R. E.
Grof, C. P. L.
Bonnett, G. D.
Maclean, D. J.
Title A bioinformatic approach to the identification of a conserved domain in a sugarcane legumain that directs GFP to the lytic vacuole
Journal name Functional Plant Biology   Check publisher's open access policy
ISSN 1445-4408
Publication date 2007
Year available 2007
Sub-type Article (original research)
DOI 10.1071/FP07024
Volume 34
Issue 7
Start page 633
End page 644
Total pages 12
Editor Henry, J.
Place of publication Australia
Publisher CSIRO Publishing
Collection year 2008
Language eng
Subject C1
270402 Plant Physiology
780105 Biological sciences
Abstract Sugarcane is an ideal candidate as a biofactory for the production of alternate higher value products. One way of achieving this is to direct useful proteins into the vacuoles within the sugarcane storage parenchyma tissue. By bioinformatic analysis of gene sequences from putative sugarcane vacuolar proteins a motif has been identified that displays high conservation across plant legumain homologues that are known to function within vacuolar compartments. This. ve amino acid motif, represented by the sequence IRLPS in sugarcane is shown to direct an otherwise secreted GFP fusion protein into a large acidic and proteolytic vacuole in sugarcane callus cells as well as in diverse plant species. In mature sugarcane transgenic plants, the stability of GFP appeared to be dependent on cell type, suggesting that the vacuolar environment can be hostile to introduced proteins. This targeting motif will be a valuable tool for engineering plants such as sugarcane for production of novel products.
Keyword Plant Sciences
biofactory
vacuole processing enzyme
Saccharum
vacuole targeting
Arabidopsis-thaliana
Plant-cells
Aspartic Proteinase
Fusion Proteins
Propeptide
Q-Index Code C1

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
School of Biological Sciences Publications
 
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