Analytical approach for selecting normalizing genes from a cDNA microarray platform to be used in q-RT-PCR assays: a cnidarian case study

Rodriguez-Lanetty, Mauricio, Phillips, Wendy S., Dove, Sophie, Hoegh-Guldberg, Ove and Weis, Virginia M. (2008) Analytical approach for selecting normalizing genes from a cDNA microarray platform to be used in q-RT-PCR assays: a cnidarian case study. Journal of Biochemical and Biophysical Methods, 70 6: 985-991.


Author Rodriguez-Lanetty, Mauricio
Phillips, Wendy S.
Dove, Sophie
Hoegh-Guldberg, Ove
Weis, Virginia M.
Title Analytical approach for selecting normalizing genes from a cDNA microarray platform to be used in q-RT-PCR assays: a cnidarian case study
Journal name Journal of Biochemical and Biophysical Methods   Check publisher's open access policy
ISSN 0165-022X
1876-7737
Publication date 2008-04-24
Year available 2007
Sub-type Article (original research)
DOI 10.1016/j.jbbm.2007.08.005
Volume 70
Issue 6
Start page 985
End page 991
Total pages 7
Editor Kilar, F.
Hjerten, S.
Place of publication Netherlands
Publisher Elsevier BV
Collection year 2008
Language eng
Subject 270201 Gene Expression
770300 Marine Environment
Abstract Research in gene function using Quantitative Reverse Transcription PCR (q-RT-PCR) and microarray approaches are emerging and just about to explode in the field of coral and cnidarian biology. These approaches are showing the great potential to significantly advance our understanding of how corals respond to abiotic and biotic stresses, and how host cnidarians/dinoflagellates symbioses are maintained and regulated. With these genomic advances, however, new analytical challenges are also emerging, such as the normalization of gene expression data derived from q-RTPCR. In this study, an effective analytical method is introduced to identify candidate housekeeping genes (HKG) from a sea anemone (Anthopleura elegantissima) cDNA microarray platform that can be used as internal control genes to normalize q-RT-PCR gene expression data. It is shown that the identified HKGs were stable among the experimental conditions tested in this study. The three most stables genes identified, in term of gene expression, were beta-actin, ribosomal protein L12, and a Poly(a) binding protein. The applications of these HKGs in other cnidarian systems are further discussed
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Published online 5 September 2007 - proof included in supporting documentation. Contined as Journal of Proteomics

Document type: Journal Article
Sub-type: Article (original research)
Collections: 2008 Higher Education Research Data Collection
Centre for Marine Studies Publications
 
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Created: Wed, 07 May 2008, 14:01:35 EST by Peter Fogarty on behalf of Centre for Marine Studies