Analytical approach for selecting normalizing genes from a cDNA microarray platform to be used in q-RT-PCR assays: A cnidarian case study

Rodriguez-Lanetty, M., Phillips, W. S., Dove, S., Hoegh-Guldberg, O. and Weis, V. M. (2008) Analytical approach for selecting normalizing genes from a cDNA microarray platform to be used in q-RT-PCR assays: A cnidarian case study. Journal of Biochemical and Biophysical Methods, 70 6: 985-991.


Author Rodriguez-Lanetty, M.
Phillips, W. S.
Dove, S.
Hoegh-Guldberg, O.
Weis, V. M.
Title Analytical approach for selecting normalizing genes from a cDNA microarray platform to be used in q-RT-PCR assays: A cnidarian case study
Journal name Journal of Biochemical and Biophysical Methods  (ERA 2012 Listed)    (ERA 2010 Rank C)   Check publisher's open access policy
Publication date 2008
Sub-type Article
Year available 2007
DOI 10.1016/j.jbbm.2007.08.005
Volume number 70
Issue number 6
ISSN 0165-022X
Start page 985
End page 991
Total pages 7
Editor Kilar, F.
Hjerten, S.
Place of publication Netherlands
Publisher Elsevier BV
Collection year 2008
Language eng
Subject 270201 Gene Expression
C1
770300 Marine Environment
Abstract Research in gene function using Quantitative Reverse Transcription PCR (q-RT-PCR) and microarray approaches are emerging and just about to explode in the field of coral and cnidarian biology. These approaches are showing the great potential to significantly advance our understanding of how corals respond to abiotic and biotic stresses, and how host cnidarians/dinoflagellates symbioses are maintained and regulated. With these genomic advances, however, new analytical challenges are also emerging, such as the normalization of gene expression data derived from q-RTPCR. In this study, an effective analytical method is introduced to identify candidate housekeeping genes (HKG) from a sea anemone (Anthopleura elegantissima) cDNA microarray platform that can be used as internal control genes to normalize q-RT-PCR gene expression data. It is shown that the identified HKGs were stable among the experimental conditions tested in this study. The three most stables genes identified, in term of gene expression, were beta-actin, ribosomal protein L12, and a Poly(a) binding protein. The applications of these HKGs in other cnidarian systems are further discussed
Q-Index Code C1
Q-Index Status Confirmed Code
Additional Notes Published online 5 September 2007 - proof included in supporting documentation

Document type: Journal Article
Sub-type: Article
Collections: 2008 Higher Education Research Data Collection
Centre for Marine Studies Publications
 
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Created: Wed, 07 May 2008, 14:01:35 EST by Peter Fogarty on behalf of Centre for Marine Studies