Diagnosis of human metapneumovirus infection in immunosuppressed lung transplant recipients and children evaluated for pertussis

Dare, Ryan, Sanghavi, Sonali, Bullotta, Arlene, Keightley, Maria-Cristina, St. George, Kirsten, Wadowsky, Robert M., Paterson, David L., McCurry, Kenneth R., Reinhart, Todd A., Husain, Shahid and Rinaldo, Charles R. (2007) Diagnosis of human metapneumovirus infection in immunosuppressed lung transplant recipients and children evaluated for pertussis. Journal of Clinical Microbiology, 45 2: 548-552. doi:10.1128/JCM.01621-06

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Author Dare, Ryan
Sanghavi, Sonali
Bullotta, Arlene
Keightley, Maria-Cristina
St. George, Kirsten
Wadowsky, Robert M.
Paterson, David L.
McCurry, Kenneth R.
Reinhart, Todd A.
Husain, Shahid
Rinaldo, Charles R.
Title Diagnosis of human metapneumovirus infection in immunosuppressed lung transplant recipients and children evaluated for pertussis
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 0095-1137
1098-660X
Publication date 2007
Year available 2006
Sub-type Article (original research)
DOI 10.1128/JCM.01621-06
Open Access Status File (Publisher version)
Volume 45
Issue 2
Start page 548
End page 552
Total pages 5
Editor A. B. Orderdonk
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Collection year 2008
Language eng
Abstract Human metapneumovirus (hMPV) is a recently discovered paramyxovirus that is known to cause respiratory tract infections in children and immunocompromised individuals. Given the difficulties of identifying hMPV by conventional culture, molecular techniques could improve the detection of this virus in clinical specimens. In this study, we developed a real-time reverse transcription-PCR (RT-PCR) assay designed to detect the four genetic lineages of hMPV. This assay and a commercial real-time nucleic acid sequence-based amplification (NASBA) assay (bioMérieux, Durham, NC) were used to determine the prevalence of hMPV in 114 immunosuppressed asymptomatic and symptomatic lung transplant recipients and 232 pediatric patients who were being evaluated for pertussis. hMPV was detected in 4.3% of the immunosuppressed lung transplant recipients and in 9.9% of children evaluated for pertussis. Both RT-PCR and NASBA assays were efficient in detection of hMPV infection in respiratory specimens. Even though hMPV was detected in a small number of the lung transplant recipients, it was still the most prevalent etiologic agent detected in patients with respiratory symptoms. In both of these diverse patient populations, hMPV infection was the most frequent viral respiratory tract infection identified. Given our findings, infection with hMPV infection should be determined as part of the differential diagnosis of respiratory illnesses.
Q-Index Code CX
Additional Notes Advance on-line publication - CX at request of school, no UQ byline

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
2008 Higher Education Research Data Collection
School of Medicine Publications
 
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Created: Tue, 06 May 2008, 10:32:45 EST by Brenda Mason on behalf of Medicine - Royal Brisbane and Women's Hospital